Yale Macaque Brain Resource

项目总结 由于非人类灵长类动物(NHP)与人类的系统发育关系密切，它的大脑 在了解人脑的结构、功能、发育和病理方面具有巨大的潜力。 然而，许多障碍阻碍了对卫生保健计划的研究(其中，重大的后勤、伦理和财政障碍 负担)。耶鲁大学神经科学系收藏了一组独特的脑组织 NHP猕猴，年龄从胚胎到成年不等，是在近50年的时间里产生的 帕斯科·拉基奇博士和已故帕特里夏·戈德曼-拉基奇博士的实验室。该集合包括多组幻灯片 用：(1)[~3H]胸腺嘧啶核苷标记的连续切片脑细胞出生测定；(2)氚标记的脑组织 用于轨迹追踪的酸；(3)有核摘除和选定皮质损伤的猴子的脑；(4)来自 出生前经X射线照射和超声照射的猴子出生后处死；(5)多个 胎儿和出生后猴子的大脑区域；以及，去年增加的，(6)组织化学和免疫组化 已加工的脑组织和(7)未加工的NHP脑组织块。 虽然MBR的过度具体目标保持不变，但已经进行了适当的调整，有利于 支持正在进行的研究项目，并扩大MBR的范围，以适应新的项目。此次续订 建议：1)继续清点、编目、恢复材料并将其添加到MBR收藏品1至4。2) 组织化学和免疫组织化学处理组织以扩展集合6并添加未处理的组织块 收集7.3)，继续宣传和传播NHP大脑信息。4)继续提供便利 资源的使用。目前续期申请的一个主要目的是维持MBR的运营和 通过为不同神经元和非神经元标记物的组织加工提供资金促进其生长(在M& 7个发育不同年龄组的标本)将被添加到收藏6。此外，脑组织 可用于不同治疗的区块将继续添加到集合7中。这笔赠款的宏伟目标是 保持不变，使这些独特的收藏成为随时可用的国际研究资源 对整个神经科学界来说。如果获得资金，MBR将继续允许和促进NHP脑研究 而不需要额外的动物祭祀。这避免了昂贵的重复实验和不必要的 使用动物。

PROJECT SUMMARY Because of its close phylogenetic relationship to the human, the non-human primate (NHP) brain holds enormous potential for understanding structure, function, development and pathology of the human brain. However, many obstacles impede studies of NHPs (among these, significant logistic, ethical, and financial burdens). The Department of Neuroscience at Yale University houses a unique collection of brain tissue from NHP macaques, ranging in age from embryonic to adult, which was generated over almost five decades in the laboratories of Dr. Pasko Rakic and the late Dr. Patricia Goldman-Rakic. The collection includes sets of slides with: (1) [3H]thymidine-labeled serially cut brains for cell birth dating; (2) brains labeled with tritiated amino acids for tract tracing; (3) brains from monkeys with enucleations and selected cortical lesions; (4) brains from prenatally X-irradiated and ultrasound-exposed monkeys sacrificed postnatally; (5) EM blocks from multiple brain regions of fetal and postnatal monkeys; and, added in the last year, (6) histo- and immunohistochemically processed brain tissue and (7) unprocessed NHP brain tissue blocks. While MBR’s overreaching Specific Aims remain the same, proper adjustments have been made, conducive to support ongoing research projects and to grow MBR in scope to accommodate new ones. This renewal proposes: 1) To continue to inventory, catalogue, restore and add materials to MBR Collections 1 to 4. 2) To histo- and immunohistochemically process tissue to expand collection 6 and to add unprocessed tissue blocks to collection 7. 3) To continue to advertise and disseminate NHP brain information. 4) To continue to facilitate use of the resource. A main purpose of the current renewal application is maintain MBR’s operations and to facilitate its growth by funding the processing of tissue for different neuronal and non-neuronal markers (in M & F specimens of 7 developmentally distinct age groups) to be added to collection 6. In addition, brain tissue blocks viable for different treatments will continue to be added to collection 7. The grand objective of this grant remains the same, to make these unique collections an international research resource that is readily available to the neuroscience community at-large. If funded, MBR will continue to permit and foster NHP brain research without requiring additional animal sacrifice. This avoids costly duplication of experiments and unnecessary use of animals.