Flow Cytometry Core

项目摘要 Moffitt癌症中心（MCC）的流式细胞术核心设施（FCC）提供集中和切割- 为MCC成员提供癌症相关研究的边缘流式细胞术服务。流式细胞术是一种 作为MCC细胞生物学、免疫学和转化研究工作不可或缺的分析工具。的目标 FCC的目标是为成员提供高技能的工作人员和先进的流式细胞术技术， 提供：1a）在单细胞水平上精确测量和分析多个参数; 1b） 能够快速纯化目标群体，评估特定的细胞功能，并从分子上表征 纯化的细胞群; 2）协助实验设计，数据分析和解释，以及 准备赠款和手稿;以及3）提供流式细胞术方面的培训和教育 技术和方法。 FCC的科学主任是Julie Djeu博士。联邦通信委员会配备了训练有素的仪器 在基于研究的流式细胞术领域拥有40多年综合经验的专家。的 该设施配备有七台台式分析仪、两台细胞分选仪、一台多重珠阵列分析仪和一台 自动珠粒分选机。FCC增加了新的仪器和升级系统，以扩大其 为会员提供服务，并提供现代流式细胞术技术。FCC使用 实验室信息管理系统（LIMS），以整合使用跟踪、调度和计费 功能协调发展的LIMS还为项目和数据管理提供了一个安全的存储库， 成员及其实验室工作人员。在此期间，FCC为171篇同行评审的出版物做出了贡献。 与前一个审查期的112个相比，审查期的112个。在最近一个财政年度，FCC提供了 服务的53名成员，与同行评审资助的成员的总使用量的89%。

PROJECT SUMMARY The Flow Cytometry Core Facility (FCC) of the Moffitt Cancer Center (MCC) provides centralized and cutting- edge flow cytometry services for MCC members for their cancer-related research. Flow cytometry is an indispensable analytical tool for cell biology, immunology, and translational research efforts at MCC. The goals of the FCC are to support members with highly skilled staff and advanced flow cytometry technologies, which provide: 1a) Accurate measurements and analyses of multiple parameters at the single-cell level; 1b) The ability to rapidly purify target populations, to assess specific cellular functions, and to molecularly characterize purified cell populations; 2) Assistance in experimental design, data analysis and interpretation, as well as with the preparation of grants and manuscripts; and 3) Delivery of training and education in flow cytometry technologies and methodologies. The Scientific Director of the FCC is Julie Djeu, PhD. The FCC is staffed with highly trained instrument specialists who have more than 40 years in combined experience in research-based flow cytometry. The facility is equipped with seven benchtop analyzers, two cell sorters, a multiplex bead array analyzer, and an automated bead sorter. The FCC has added new instrumentation and upgraded systems to expand its services to members and to provide access to modern flow cytometry technologies. The FCC employs the use of a Laboratory Information Management System (LIMS) to consolidate usage tracking, scheduling, and billing functions. The LIMS also provides a secure repository for project and data management, which is accessible by members and their laboratory staff. The FCC has contributed to 171 peer-reviewed publications during this review period compared with 112 the previous review period. In the most recent fiscal year, the FCC provided service for 53 members, with 89% of total usage for peer-review-funded members.