NeMO Archive: SCORCH Support, Coordination and Outreach

摘要 我们建议建立一个数据协调中心来分析产生的单细胞和其他分子数据集 在艾滋病毒(SCOCH)和其他NIDA资助的艾滋病毒和物质的背景下的单细胞阿片反应 使用无序项目。该项目将利用我们的神经科学多组体数据档案，它将托管大多数 大脑主动的多组数据(NEMO档案；R24MH114788)，并代表了现有的几个关键 由华盛顿大学开发的数据管理、集成和网络演示工具的资源 马里兰集团。该项目的首席调查员、协查人员和工作人员具有不同的专业知识 需要在整个SCARCH项目财团中整理数据，包括从 多个机构，大规模的质量控制和分析处理能力，熟悉NIH政策， 以及对公共档案存放策略的直接经验。活动将围绕尼莫组织 档案馆，一个专门专注于存储和传播来自 大脑倡议和相关的大脑研究项目。我们将使用联合模型进行数据存储，例如 数据的物理位置可以在NEMO本地文件系统、公共存储库 和远大云计算环境。NEMO-SCOCH数据中心将完全与 在下一代NIH推出资源的研究社区成员提出的原则 数据生态系统。这些做法包括公平原则、API文档、数据索引系统、 工作流共享、可共享软件管道的使用以及基于云的系统上的集中存储。这个 将信息并入NEMO-SCOCH数据中心将在一定程度上帮助理解1) 与脑异常和疾病相关的基因组区域；2)转录因子结合位点和 其他调控元件；3)转录活性；4)胞嘧啶修饰水平；5)组蛋白 修饰轮廓和染色质可及性。

SUMMARY We propose to set up a data coordination center to analyze single cell and other molecular data sets generated by Single Cell Opioid Responses in the Context of HIV (SCORCH) and other NIDA-funded HIV and substance use disorder projects. This project will leverage our Neuroscience Multi-Omic Data Archive which will host most of the BRAIN initiative multi-omic data (NeMO Archive; R24MH114788) and represents several key existing resources for data management, integration and web presentation tools developed by the University of Maryland group. The Principle Investigator, Co-investigators, and staff of this project have diverse expertise required to marshall data across the SCORCH project consortium, including experience in data collection from multiple institutions, large-scale quality control and analysis processing capability, familiarity with NIH policy, and direct experience with public archive deposition strategies. Activities will be organized around the NeMO Archive, a data repository that is specifically focused on the storage and dissemination of `omic data from the BRAIN Initiative and related brain research projects. We will utilize a federated model for data storage such that the physical location of data can be distributed between the NeMO local file system, public repositories, and the Broad cloud computing environment. The NeMO-SCORCH data center will be fully consistent with the principles advanced by research community members who are launching resources in next generation NIH data ecosystem. These practices include FAIR Principles, documentation of APIs, data-indexing systems, workflow sharing, use of shareable software pipelines, and centralized storage on cloud-based systems. The information incorporating into the NeMO-SCORCH data center will, in part, enable understanding of 1) genomic regions associated with brain abnormalities and disease; 2) transcription factor binding sites and other regulatory elements; 3) transcription activity; 4) levels of cytosine modification; and 5) histone modification profiles and chromatin accessibility.