The interaction between vocal fold hydration and vibratory biomechanics

项目摘要/摘要 嗓音障碍影响着全球数百万人。患者报告了许多负面的社会问题 发音困难的经济影响。适当的体液平衡对正常的声带功能至关重要。维修 声带组织中的正常液体水平确保了正常的生物力学和振动参数。潜力 对这种动态平衡的干扰包括过度使用、滥用、全身或表面脱水、创伤和 发炎。这些扰动的结果可能导致应力分布的改变，机械损伤， 和炎症。组织的进一步损伤可能会导致水肿或良性病变的形成。 这项建议的总体目标是评估声带液体动态平衡的贡献， 组织特性和振动生物力学导致组织损伤并最终导致水肿。结果将会是 解释发声过程中振动力学的变化。流体的量化方法 本提案中开发的体外和活体模型中的内容将提供有关流体的必要信息 内容和加深我们对液体在声带振动中的作用的认识和理解。知识 这项研究对于更全面地理解嗓音的临床处理是必不可少的 精神错乱。 这项研究的方法将涉及三个具体目标。第一个目标将集中在方法上 量化体液含量和组织属性。我们将把四种新技术应用到喉科：(1)组织 介电特性(TDP)，测量组织水分含量；(2)光学相干层析成像(OCT)作为 定量组织中液体体积的方法；(3)激光多普勒血流计，一种测量通过组织的血液流量的方法 组织；以及(4)声弹性图，这是一种测量组织的声学特性的方法，它与 应变与组织应力呈非线性相关。这些方法的结果将有助于建立 间质液体水平和组织特性的标准。第二个目标将侧重于评估 振动对声带内液体含量和动力学的影响。首先，采用有限元建模的方法进行研究 不同伸长率、声门下压力、毛细血管时振动对液体含量和动力学的影响 渗透性和硬度。模型趋势的生理学验证将在离体动物身上进行。 模特们。最后，我们将使用一个因长时间振动而引起炎症的动物模型来观察 过度使用发声时可能会出现水肿。第三个目标将集中在液体含量对声带的影响上。 震动。我们将确定声带病理改变时的水合程度。在其他 当发声参数(如发声门槛压力)发生显著变化时。 在这一目的中，将使用体外模型来研究脱水和过度水化的生物力学效应。在……里面 此外，声弹声图将被用来研究组织的应力分布的变化 流体含量的变化。

Project Summary/Abstract Voice disorders affect millions of people worldwide. Patients have reported many negative social econmic impacts of dysphonia. Proper fluid homeostasis is critical to normal vocal fold function. Maintenance of normal fluid levels in vocal fold tissue ensures normal biomechanical and vibratory parameters. Potential disturbances to this homeostasis include overuse, misuse, systemic or surface dehydration, trauma, and inflammation. The results of these disturbances can lead to changes in stress distribution, mechanical damage, and inflammation. Further damage to the tissue might result in the formation of edema or a benign lesion. The overall goal of this proposal is to evaluate the contributions of vocal fold fluid homeostasis, tissue properties, and vibratory biomechanics to tissue damage and ultimately edema. The results will explain changes in vibration mechanics over extended periods of phonation. Methods of quantifying fluid content in excised and in vivo models developed in this proposal will provide necessary information on fluid content and further our knowledge and understanding of the role of fluid in vocal fold vibration. Knowledge gained from this research is essential for a more complete understanding of clinical management of voice disorders. The approach to this research will involve three specific aims. The first aim will focus on methods of quantifying fluid content and tissue properties. We will employ four technologies novel to laryngology: (1) tissue dielectric properties (TDP), a measure of tissue water content; (2) Optical Coherence Tomography (OCT) as a method to quantify fluid volume in tissues; (3) laser Doppler flowmetry, a measure of blood flow through a tissue; and (4) acoustoelastography, a measure of the acoustic properties of the tissue that is linearly related to strain and nonlinearly related to tissue stress. The results of these methods will be useful for establishing standards in interstitial fluid levels and tissue properties. The second aim will focus on evaluating the effects of vibration on fluid content and dynamics in the vocal folds. First, finite element modeling will be used to study the effects of vibration on fluid content and dynamics while varying elongation, subglottal pressure, capillary permeability and stiffness. Physiological validation of the model trends will be carried out on excised animal models. Finally, an animal model of inflammation due to prolonged vibration will be used to observe how edema might occur in vocal overuse. The third aim will focus on the effects of fluid content on vocal fold vibration. We will determine hydration levels at which the vocal folds are pathologically changed. In other words, when there is a significant change to phonation parameters such as phonation threshold pressure. Excised models will be used in this aim to study the biomechanical effects of dehydration and overhydration. In addition, acoustoelastography will be employed to study the change in stress distribution of the tissue with changes in fluid content.