Oklahoma Nathan Shock Center of Excellence in Basic Biology of Aging

俄克拉荷马州休克中心内森休克中心旨在提供独特的、创新的服务 目前在大多数机构或其他休克中心都有。中心的重点是发展 年轻研究人员的职业生涯，但我们的服务是整个研究社区都可以使用的 老龄化与老年科学。俄克拉荷马州休克中心的独特之处在于，它专注于老年科学和 得到了俄克拉荷马城三大研究机构的机构支持：俄克拉荷马大学 健康科学中心、俄克拉何马州医学研究基金会和俄克拉何马城退伍军人医学中心。 在过去四年，超过75%的试验补助金由研究发展核心和 研究中心向研究人员提供的所有服务中，有近40%是在 主办机构以外的其他机构。2020年俄克拉荷马州内森冲击中心有六个核心。这个 计划增强核心监督休克中心的所有活动，并组织年度 俄克拉荷马州老年科学研讨会，为研究生和来自其他地区的研究员提供旅行奖励 参加研讨会的机构。核心中的老年病理学研究资源为研究人员提供 用老年病理学评分平台对小鼠进行病理评估。研究进展 核心是对内森冲击中心类似核心的传统结构的创新，并是 旨在为全国科学界提供前所未有的服务。《多工》 蛋白质分析核心为研究人员提供了测量丰度的新的质谱学方法 来自任何生物的任何组织/细胞的蛋白质，这些蛋白质的基因组已经被测序。核心可以量化面板 在一次运行中检测到50种蛋白质。使用D2O标签，核心还可以测量合成和周转 这些蛋白质。结合起来，这些分析有助于询问蛋白质组在通路中的变化 对老龄化和老年科学具有重要意义。基因组科学核心已经开发出测试方法来测量 DNA在基因组特定位置的甲基化和羟甲基化，这将是 可供研究衰老表观遗传学的研究人员使用。核心还为研究人员提供了 线粒体基因组异质性(变异/突变和缺失)和复制的综合分析 数量分析和单细胞转录组分析。老年科学氧化还原生物学核心提供 研究人员测量氧化损伤、氧化还原对和线粒体功能，这些指标 需要专门的仪器和专业知识。核心还为调查人员提供了最新的 啮齿动物体内和原位组织中的活性氧测定技术。这个 GeroInformatics Core为研究人员提供了分析大数据集、统计 支持、分析高通量数据集，包括单细胞转录数据和HAS 开发了独特的生物信息学工具和方法来帮助调查人员发现新的联系 在他们的数据和年龄相关现象之间。

The Oklahoma Shock Nathan Shock Center is designed to deliver unique, innovative services that are not currently available at most institutions or at other Shock Centers. The focus of the Center is on the development of careers of young investigators but our services are accessible by the entire research community studying aging and Geroscience. The Oklahoma Shock Center is unique in that it focuses on the field of Geroscience and has institutional support from the three major research institutions in Oklahoma City: the University of Oklahoma Health Science Center, the Oklahoma Medical Research Foundation, and the Oklahoma City VA Medical Center. Over the past four years, more than 75% of the Pilot Grants awarded by the Research Development Core and almost 40% of all services provided researchers by the Research Cores have been provided to investigators at institutions other than the host institutions. The 2020 Oklahoma Nathan Shock Center has six Cores. The Program Enhancement Core oversees all the activities of the Shock Center and organizes the annual Oklahoma GeroScience Symposium, which provides travel awards to graduate students and fellows from other institutions to attend the Symposium. The Geropathology Research Resource in the Core provides investigators with pathological assessments of mice using the Geropathology Grading Platform. The Research Development Core is an innovative departure from the traditional structure of similar Cores in Nathan Shock Centers and is designed to provide unprecedented service to the scientific community throughout the nation. The Multiplexing Protein Analysis Core provides investigators novel mass spectrometry methods for measuring the abundance of proteins from any tissue/cell from any organism that has its genome sequenced. The Core can quantify panels of ~50 proteins in a single run. Using D2O labeling, the Core can also measure the synthesis and turnover of these proteins. In combination, these analyses facilitate interrogation of proteomic changes in pathways of importance to aging and Geroscience. The Genomic Sciences Core has developed assays to measure both the methylation and hydroxymethylation of DNA at specific sites in the genome, which will be available to investigators studying epigenetics of aging. The Core also offers researchers the comprehensive analysis of mitochondrial genome heteroplasmy (variants/mutations and deletions) and copy number as well as single-cell transcriptomics assays. The Geroscience Redox Biology Core provides investigators with measures of oxidative damage, redox couples, and mitochondrial function, which require specialized instrumentation and expertise. The Core also provides investigators with the latest technology to measure reactive oxygen species in vivo and in situ in tissues of rodents. The GeroInformatics Core provides investigators assistance in the analysis of large datasets, statistical support, analysis of high-throughput datasets including single-cell transcriptomic data and has developed unique bioinformatics tools and methods to assist investigators to discover new connections between their data and age-related phenomenon.