Oklahoma Nathan Shock Center of Excellence in Basic Biology of Aging

俄克拉荷马州内森休克衰老基础生物学卓越中心

• 批准号: 10649612
• 负责人: Veronica Galvan
• 金额: $ 106.06万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10649612
• 关键词: Address; Aging; Award; Bioinformatics; Biological Assay; Biology; Biology of Aging; Cell Culture Techniques; Cells; Centers of Research Excellence; Cities; Communities; Country; Couples; DNA; Data; Data Analyses; Data Set; Development; Disease; Education; Environment; Epigenetic Process; Equipment; Faculty; Foundations; Free Radicals; Funding; Future; Generations; Genes; Genome; Geroscience; Goals; Grant; Health Sciences; Human; Image; In Situ; Individual; Institution; Intervention; Label; Mass Spectrum Analysis; Measurement; Measures; Medical Research; Medical center; Mentors; Methods; Methylation; Mitochondria; Modeling; Molecular Target; Mus; Mutation; Oklahoma; Organism; Oxidation-Reduction; Pathologic; Pathway interactions; Population; Production; Protein Analysis; Protein Biosynthesis; Proteins; Proteomics; Rattus; Reactive Oxygen Species; Research; Research Personnel; Resources; Risk Factors; Rodent; Running; Sampling; Services; Shock; Site; Structure; Techniques; Technology; Tissues; Training; Travel; United States; Universities; Variant; age effect; age related; animal tissue; bioinformatics tool; career; career development; design; genome sciences; genome-wide; graduate student; heteroplasmy; high throughput analysis; in vivo; innovation; instrumentation; large datasets; mitochondrial genome; next generation sequencing; novel; novel strategies; outreach; oxidative damage; programs; protein degradation; recruit; research and development; symposium; transcriptomics

The Oklahoma Shock Nathan Shock Center is designed to deliver unique, innovative services that are not currently available at most institutions or at other Shock Centers. The focus of the Center is on the development of careers of young investigators but our services are accessible by the entire research community studying aging and Geroscience. The Oklahoma Shock Center is unique in that it focuses on the field of Geroscience and has institutional support from the three major research institutions in Oklahoma City: the University of Oklahoma Health Science Center, the Oklahoma Medical Research Foundation, and the Oklahoma City VA Medical Center. Over the past four years, more than 75% of the Pilot Grants awarded by the Research Development Core and almost 40% of all services provided researchers by the Research Cores have been provided to investigators at institutions other than the host institutions. The 2020 Oklahoma Nathan Shock Center has six Cores. The Program Enhancement Core oversees all the activities of the Shock Center and organizes the annual Oklahoma GeroScience Symposium, which provides travel awards to graduate students and fellows from other institutions to attend the Symposium. The Geropathology Research Resource in the Core provides investigators with pathological assessments of mice using the Geropathology Grading Platform. The Research Development Core is an innovative departure from the traditional structure of similar Cores in Nathan Shock Centers and is designed to provide unprecedented service to the scientific community throughout the nation. The Multiplexing Protein Analysis Core provides investigators novel mass spectrometry methods for measuring the abundance of proteins from any tissue/cell from any organism that has its genome sequenced. The Core can quantify panels of ~50 proteins in a single run. Using D2O labeling, the Core can also measure the synthesis and turnover of these proteins. In combination, these analyses facilitate interrogation of proteomic changes in pathways of importance to aging and Geroscience. The Genomic Sciences Core has developed assays to measure both the methylation and hydroxymethylation of DNA at specific sites in the genome, which will be available to investigators studying epigenetics of aging. The Core also offers researchers the comprehensive analysis of mitochondrial genome heteroplasmy (variants/mutations and deletions) and copy number as well as single-cell transcriptomics assays. The Geroscience Redox Biology Core provides investigators with measures of oxidative damage, redox couples, and mitochondrial function, which require specialized instrumentation and expertise. The Core also provides investigators with the latest technology to measure reactive oxygen species in vivo and in situ in tissues of rodents. The GeroInformatics Core provides investigators assistance in the analysis of large datasets, statistical support, analysis of high-throughput datasets including single-cell transcriptomic data and has developed unique bioinformatics tools and methods to assist investigators to discover new connections between their data and age-related phenomenon.

俄克拉荷马州冲击内森冲击中心旨在提供独特的，创新的服务， 目前在大多数机构或其他休克中心提供。该中心的重点是发展 年轻研究人员的职业生涯，但我们的服务是由整个研究界研究 老龄化和老年科学俄克拉荷马州休克中心的独特之处在于，它专注于老年科学领域， 有来自俄克拉荷马州市三大研究机构的机构支持：俄克拉荷马州大学 健康科学中心、俄克拉荷马州医学研究基金会和俄克拉荷马州市退伍军人医疗中心。 在过去四年，研究发展核心计划批出的试验赠款， 研究中心向研究人员提供的所有服务中，近40%是在 除主办机构外的其他机构。2020年俄克拉荷马州内森休克中心有六个核心。的 计划增强核心监督休克中心的所有活动，并组织年度 俄克拉荷马州老年科学研讨会，该研讨会为来自其他国家的研究生和研究员提供旅行奖励。 参加研讨会的机构。核心中的老年病理学研究资源为研究人员提供了 使用老年病理学分级平台对小鼠进行病理学评估。研究进展 核心是一个创新的出发点，从传统的结构类似的核心在内森休克中心， 旨在为全国科学界提供前所未有的服务。复用 蛋白质分析核心为研究人员提供了新的质谱方法来测量丰度 任何组织/细胞中的蛋白质，任何生物体的基因组测序。核心可以量化面板 一次运行约50种蛋白质。使用D2 O标记，核心还可以测量合成和营业额 这些蛋白质。结合起来，这些分析促进了对通路中蛋白质组变化的询问。 对老龄化和老年科学的重要性。基因组科学中心已经开发出检测方法， 基因组中特定位点的DNA甲基化和羟甲基化，这将是 提供给研究衰老表观遗传学的研究人员。核心还为研究人员提供了 线粒体基因组异质性（变体/突变和缺失）和拷贝的综合分析 数量以及单细胞转录组学测定。Geroscience Redox Biology Core提供 研究人员测量氧化损伤，氧化还原对和线粒体功能， 需要专门的仪器和专业知识。核心还为调查人员提供最新的 啮齿类动物体内和组织原位活性氧测定技术。的 GeroInformatics Core为调查人员提供大型数据集分析，统计 支持，分析高通量数据集，包括单细胞转录组数据， 开发了独特的生物信息学工具和方法，以帮助研究人员发现新的联系 他们的数据和年龄相关的现象。

项目成果

Sex Differences in the Skeletal Muscle Response to a High Fat, High Sucrose Diet in Rats.

• DOI: 10.3390/nu15204438
• 发表时间: 2023-10-19
• 期刊: Nutrients
• 影响因子: 5.9
• 作者: Hulett NA;Knaub LA;Hull SE;Pott GB;Peelor R;Miller BF;Shankar K;Rudolph MC;Reusch JEB;Scalzo RL
• 通讯作者: Scalzo RL

Absence of Either Ripk3 or Mlkl Reduces Incidence of Hepatocellular Carcinoma Independent of Liver Fibrosis.

• DOI: 10.1158/1541-7786.mcr-22-0820
• 发表时间: 2023-09-01
• 期刊: MOLECULAR CANCER RESEARCH
• 影响因子: 5.2
• 作者: Mohammed, Sabira;Thadathil, Nidheesh;Ohene-Marfo, Phoebe;Tran, Albert L.;Veldt, Michael Van Der;Georgescu, Constantin;Oh, Sangphil;Nicklas, Evan H.;Wang, Dawei;Haritha, Nair Hariprasad;Luo, Wenyi;Janknecht, Ralf;Miller, Benjamin F.;Wren, Jonathan D.;Freeman, Willard M.;Deepa, Sathyaseelan S.
• 通讯作者: Deepa, Sathyaseelan S.

Endothelial dysfunction and angiogenesis impairment in the ageing vasculature.

• DOI: 10.1038/s41569-018-0030-z
• 发表时间: 2018-09
• 期刊: Nature reviews. Cardiology
• 作者: Ungvari Z;Tarantini S;Kiss T;Wren JD;Giles CB;Griffin CT;Murfee WL;Pacher P;Csiszar A
• 通讯作者: Csiszar A

An unbiased seed-based RNAi selection screen identifies small RNAs that inhibit androgen signaling and prostate cancer cell growth.

• DOI: 10.1016/j.omtn.2023.06.021
• 发表时间: 2023-09-12
• 期刊: MOLECULAR THERAPY NUCLEIC ACIDS
• 作者: Corbin, Joshua M.;Georgescu, Constantin;Wang, Lin;Wren, Jonathan D.;Bieniasz, Magdalena;Xu, Chao;Asch, Adam S.;Echevarria, Maria J. Ruiz
• 通讯作者: Echevarria, Maria J. Ruiz

A Genetically Heterogeneous Rat Model with Divergent Mitochondrial Genomes.

• DOI: 10.1093/gerona/glad056
• 发表时间: 2023-05-11
• 期刊: JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
• 影响因子: 5.1
• 作者: Sathiaseelan, Roshini;Ahn, Bumsoo;Stout, Michael B.;Logan, Sreemathi;Wanagat, Jonathan;Nguyen, Hoang Van M.;Hord, Norman G.;Vandiver, Amy R.;Selvarani, Ramasamy;Ranjit, Rojina;Yarbrough, Hannah;Masingale, Anthony;Miller, Benjamin F.;Wolf, Roman F.;Austad, Steven N.;Richardson, Arlan
• 通讯作者: Richardson, Arlan