BioRepository and Precision Pathology Center

摘要-生物资源库和精密病理学中心共享资源 BioRepository & Precision Pathology Center（BRPC）提供基于患者组织和血液的研究 杜克癌症研究所（DCI）。主要服务领域是生物库（BRPC广泛同意，组织， 血液和液体样本的采集、处理、储存和分发，包括BRPC患者的血液和液体样本的采集、处理、储存和分发- 来源的异种移植（PDX）和原代细胞培养（PCC）服务线，通过 宏观/微观解剖，DNA/RNA提取），监管（方案审查，工作范围创建，预算编制， 如果可能，在广泛同意的情况下整合）和免疫组织学（传统组织学，单一和多重 免疫组织化学、原位杂交、组织微阵列产生、全载玻片成像和图像分析）。 关于生物库服务，BRPC管理杜克最大的样本广泛同意协议 收集用于研究，这正在成为新的微生物组倡议的主要同意， 自2012年以来，有超过4，700名患者同意。在这些患者中，17%是非洲裔美国人，4%是其他非裔美国人。 高加索人，支持对服务不足的少数民族的癌症研究。BRPC还设有一所 美国病理学家（CAP）认可的正常和患病样本的生物储存库，代表了一系列 实体和血淋巴病理。银行业务活动与抽血、标准- 护理活检和外科手术以及临床试验支持的组织采集。BRPC优先考虑活动 根据DCI的疾病现场小组领导人的指示。通常一个生物库事件可以同时 提供多项正在进行的研究工作，准备PDX模型，准备PCC，并为未来储备生物样本库 需求BRPC生物标本也被用于NCI的CPTAC项目的第三阶段。BRPC还提供 作为DCI个性化癌症医学倡议（PCMI）的行政和监管骨干， 其将基因组信息、临床注释和生物样本连接起来。通往石蜡组织的通道 病理学系的档案，BRPC提供监管支持，队列识别， 使用这些档案进行回顾性研究的病理学家审查。BRPC快速检索和准备患者的 用于提交给临床试验申办者的石蜡存档组织样本。最后，BRPC提供专门的 组织处理和实验服务，包括基础组织学，动物兽医病理学支持 模型，免疫组织化学，原位杂交分析，组织微阵列创建，肿瘤富集， 显微切割和激光捕获显微切割、整个切片成像和图像分析算法。BRPC 贡献给癌症基因组图谱项目的生物标本支持了许多高影响力的 发表在Cell、Nature、Cancer Cell和Cell Reports上。 2018年，BRPC为177名调查人员提供了服务，其中58%是DCI成员， 占总使用量的92%，代表了所有8个DCI研究项目。DCI成员使用BRPC， 在本项目期间，为170份出版物做出了贡献，其中54份发表在影响力大的期刊上。

ABSTRACT – BIOREPOSITORY & PRECISION PATHOLOGY CENTER SHARED RESOURCE The BioRepository & Precision Pathology Center (BRPC) provides patient tissue and blood-based research support for the Duke Cancer Institute (DCI). Major service areas are Biobanking (BRPC broad consent, tissue, blood and fluid sample procurement, processing, storage and distribution including for the BRPC’s patient- derived xenograft (PDX) and primary cell culture (PCC) service lines, tumor enrichment through macro/microdissection, DNA/RNA extraction), Regulatory (protocol review, scope of work creation, budgeting, integration with broad consent if possible), and ImmunoHistology (traditional histology, single and multiplexed immunohistochemistry, in situ hybridization, tissue microarray creation, whole slide imaging and image analysis). Regarding the Biobanking service, the BRPC administers Duke’s largest broad consent protocol for specimen collection for research, which is becoming the backbone consent for new microbiome initiatives and has consented >4,700 patients since 2012. Of these patients, 17% are African American and 4% other non- Caucasian, supporting research into cancers of underserved minorities. The BRPC also operates a College of American Pathologists’ (CAP)-accredited biorepository of normal and diseased samples representing a range of solid and hematolymphoid pathologies. Banking activities occur in conjunction with blood draws, standard-of- care biopsy and surgical procedures and clinical trial-supported tissue acquisitions. BRPC prioritizes activities based on instructions of DCI’s disease-site group leaders. Often a single biobanking event can simultaneously supply multiple ongoing research efforts, prepare a PDX model, prepare PCCs, and stock the biobank for future needs. BRPC biospecimens also are being used in Phase III of the NCI’s CPTAC project. The BRPC also serves as the administrative and regulatory backbone for the DCI’s Personalized Cancer Medicine Initiative (PCMI), which links genomic information, clinical annotation, and biospecimens. The gateway to the paraffin tissue archives in the Department of Pathology, BRPC provides regulatory support, cohort identification, and pathologist review for retrospective studies using those archives. BRPC rapidly retrieves and prepares patients’ paraffin archival tissue samples for submission to clinical trial sponsors. Finally, the BRPC provides specialized tissue processing and experimental services including basic histology, veterinary pathology support for animal models, immunohistochemistry, in situ hybridization assays, tissue microarray creation, tumor enrichment by microdissection and laser capture microdissection, whole slide imaging and image analysis algorithms. BRPC biospecimens contributed to the Cancer Genome Atlas Project have supported numerous high-impact publications in Cell, Nature, Cancer Cell, and Cell Reports. In 2018, the BRPC provided services to 177 investigators, 58% of whom were DCI members who accounted for 92% of total usage and represented all 8 DCI Research Programs. Use of BRPC by DCI Members, contributed to 170 publications over the current project period, of which 54 were in high impact journals.