Single-Cell Biology Shared Resource

单细胞生物共享资源--项目摘要/摘要 单细胞生物学共享资源成立于2017年，以开拓和部署为使命 为CSHL癌症中心和周边组织成员提供尖端单细胞基因组学技术 研究社区。共享资源由系主任杰西·吉利斯博士和主任乔纳森领导 单细胞生物学共享资源被设计为一个全面的、协作性的共享资源 支持单细胞基因组计划每个阶段的资源设施，包括实验设计， 协议制定、样品制备、文库建设、测序后勤、初级数据处理、 和深入的嵌入式分析。共享资源人员经常与研究人员并肩工作， 自定义工作流以满足项目的独特需求。最常见的单电池示例 共享资源支持的工作流程包括：3‘-数字转录组分析、免疫受体 图谱分析、ATAC测序、拷贝数变异、蛋白质组学和单核RNAseq。除了……之外 共享资源是标准的、已建立的协议，可帮助用户设计和执行定制的单一 具有下游定制数据分析的单元工作流。单细胞生物学共享资源库和 运营资本设备，用于处理常见的单单元工作流的大多数阶段。的关键组件 该共享资源的使命是提供单细胞基因组数据分析方面的教育和培训。 半年一次的研讨会和定期的计算办公时间为研究人员提供了学习的机会 并熟悉数据分析中的新兴技术。因为它的 自成立以来，共享资源已被12个CSHL癌症中心实验室使用(占成员的32%)， 结果出版了5本合著的出版物。总之，单细胞生物学共享资源提供了 对现成和定制的基因组学工作流程提供全面的工作台到出版支持， 极大地简化了收集这些具有挑战性和高影响的数据集的过程。

SINGLE-CELL BIOLOGY SHARED RESOURCE – PROJECT SUMMARY/ABSTRACT The Single-Cell Biology Shared Resource was founded in 2017 with the mission of pioneering and deploying cutting-edge single-cell genomics technologies to members of the CSHL Cancer Center and surrounding research community. The Shared Resource is led by Faculty Head Jesse Gilis, Ph.D., and Director Jonathan Preall, Ph.D. The Single-Cell Biology Shared Resource is designed to be a comprehensive, collaborative Shared Resource facility that supports every stage of a single-cell genomics project, including experimental design, protocol development, sample preparation, library construction, sequencing logistics, primary data processing, and in-depth embedded analysis. Shared Resource personnel frequently work side-by-side with researchers, customizing the workflow to meet the unique needs of the project. Examples of the most common single-cell workflows supported by the Shared Resource include: 3’-digital transcriptome profiling, immune receptor profiling, ATAC-sequencing, copy-number variation, proteogenomics, and single-nucleus RNAseq. In addition to standard, established protocols, the Shared Resource assists users in designing and executing custom single- cell workflows with downstream tailored data analysis. The Single-Cell Biology Shared Resource houses and operates capital equipment for handling most stages of common single-cell workflows. A critical component of this Shared Resource’s mission is to provide education and training on the analysis of single-cell genomics data. Semi-annual workshops and regular computational office hours give researchers the opportunity to learn common computational pipelines and become familiar with emerging techniques in data analysis. Since its establishment, the Shared Resource has been used by 12 CSHL Cancer Center laboratories (32% of members), resulting in 5 co-authored publications. In summary, the Single-Cell Biology Shared Resource provides comprehensive bench-to-publication support of both off-the-shelf and customized genomics workflows, dramatically streamlining the process of collecting these challenging and high-impact datasets.