miRNA profiling of prostate cancer in young and older male patients

我们的目的是研究年轻患者前列腺肿瘤的miRNA谱，并与老年患者前列腺癌的miRNA表达进行比较。我们将评估不同分化程度和不同生物学行为的肿瘤的特异性miRNA表达模式是否存在差异。前列腺癌将被手工显微解剖，以获得肿瘤细胞的纯群体，以及可用的正常上皮远离肿瘤区域。组织学特征如Gleason评分、有无神经周围、淋巴浸润、囊外或精囊浸润也被评估和记录。差异表达的mirna将被定义为具有两倍变化（向上或向下）且p值为0.05的mirna。不同的miRNAs表达谱将包括低级别肿瘤（Gleason评分6和7）与高级别肿瘤（Gleason评分8和9）病变之间的比较。然后，将每个肿瘤组与各自组的正常上皮进行比较。研究将选择年龄在40-50岁及以上的患者。

Our goal is to investigate the miRNA profiles of prostate tumors occurring in young patients and compare with the miRNA expression of prostate cancers occurring in the elderly patients. We will evaluate if there are differences in specific miRNA expression patterns of tumors with different grades of differentiation and different biological behavior. Prostate cancers will be manually microdissected to obtain pure populations of tumor cells as well as available normal epithelium distant to the tumor area. Histological features such as Gleason score, presence of perineural, lymphatic invasion, extracapsular or seminal vesicle invasion were also evaluated and recorded for each case. Differentially expressed miRNAs will be defined as those with a two-fold change (up or down) and a p value 0.05. Different miRNAs expression profiles will include comparison between the low grade tumors (Gleason score 6 and 7) vs. high grade (Gleason score 8 and 9) lesions. Then, each tumor group will be compared with their respective group of normal epithelium. Patients of younger 40-50 years and older will be selected for the study.