miRNA profiling of prostate cancer.

我们的目标是研究前列腺肿瘤和正常上皮的miRNA谱，以评估不同分化程度和不同生物学行为的肿瘤是否存在特定的miRNA表达模式的差异。前列腺癌将被手动显微解剖，以获得纯净的肿瘤细胞群体以及远离肿瘤区域的可用的正常上皮。对每个病例的组织学特征，如Gleason评分、神经周围病变、淋巴管侵犯、囊外或精囊侵犯等进行评估和记录。差异表达的miRNA将被定义为具有两倍变化(向上或向下)和p值的miRNAs。不同的miRNAs表达谱将包括低级别肿瘤(Gleason评分6和7)与高级别肿瘤(Gleason评分8和9)之间的比较。然后，将每个肿瘤组与各自的正常上皮组进行比较。

Our goal is to investigate the miRNA profiles of prostate tumors and the normal epithelium to evaluate if there are differences in specific miRNA expression patterns of tumors with different grades of differentiation and different biological beavior. Prostate cancers will be manually microdissected to obtain pure populations of tumor cells as well as available normal epithelium distant to the tumor area. Histological features such as Gleason score, presence of perineural, lymphatic invasion, extracapsular or seminal vesicle invasion were also evaluated and recorded for each case. Differentially expressed miRNAs will be defined as those with a two-fold change (up or down) and a p value <0.05. Different miRNAs expression profiles will include comparison between the low grade tumors (Gleason score 6 and 7) vs. high grade (Gleason score 8 and 9) lesions. Then, each tumor group will be compared with their respective group of normal epithelium.