Developmental origins and homeostatic mechanisms underlying adult phenotypes: multispectral sorting of pigment cells from zebrafish and non-traditional model species

联系PD/PI：PARICHY，David M 补充要求摘要 本课题组主要研究了色素细胞类型和色素模式变异fi阳离子的机制 在fi中，她属于danio属，包括斑马fish d.rerio，以及更远的近亲。 物种。本设备补充申请寻求购买Cytek Aurora CSfl发光激活电池 这将极大地增强我们实现奖项最初目标的能力，这需要广泛的罪过- 斑马fish和突变背景下野生型和突变型色素细胞的Gle细胞序列分析 其他Danios，一种被证明在技术上难以解决的方法，以及fi在纳米上不可行的指令- 当前可用的MET。Cytek Aurora CS还将允许我们采用创新的体外方法来 阐明斑马fish在图案形成过程中细胞-细胞相互作用的机制，同时独特地允许 美国将使用海洋fifiSHES测试与细胞类型进化多样化有关的新假说(例如， finfenmaa)和其他物种(如Rasbora trrigonostigma Heteroma)不能 在实验室中培育，缺乏斑马fish的遗传和基因组资源。 1 项目摘要/摘要第6页

Contact PD/PI: PARICHY, DAVID M SUMMARY OF SUPPLEMENT REQUEST The parent project focuses on elucidating mechanisms of pigment cell type and pigment pattern diversification in the fishes of the genus Danio, which includes the zebrafish D. rerio, as well as more distantly related species. This equipment supplement request seeks purchase of a Cytek Aurora CS fluorescence activated cell sorter that will greatly enhance our ability to meet the original goals of the award, which require extensive sin- gle cell RNA-sequencing analyses of pigment cells from wild-type and mutant backgrounds of zebrafish and other danios, an approach that is proving to be technically intractable and financially infeasible with the instru- ment currently available. The Cytek Aurora CS will also permit us to take an innovative, in vitro approach to elucidating mechanisms of cell-cell interactions during pattern formation in zebrafish, while uniquely allowing us to test new hypotheses pertaining to evolutionary diversification of cell types using marine fishes (e.g., the wrasse Cirrhilabrus finfenmaa) and other species (e.g., rasbora Trigonostigma heteromorpha) that cannot be bred in the laboratory and that lack the genetic and genomic resources of zebrafish. 1 Project Summary/Abstract Page 6