Animal and Biostatistics Core

摘要 动物和生物统计学核心将直接与项目负责人合作，设计、计划、监测和解释 所有的动物实验，以及，提供特定的小鼠模型和标准化的实验方案， 支持整个计划的共同目标：1）了解年龄和遗传背景如何调节 禁食模拟饮食（FMD）和蛋白质限制循环（PRC）对寿命和健康的影响，2） 研究FMD，PRC依赖的细胞保护，再生和复壮的机制， 造血和神经系统，3）了解FMD与PRC，GH-IGF-1，mTOR和 humanin来检验这一假设，即这种线粒体肽是PFC/PRC介导剂， 作为禁食/蛋白质限制模拟物，4）研究半胱氨酸γ裂解酶依赖性 气体H2S的产生，由GH/IGF-1-mTOR负调控，并检验H2S是一种 FMD对肝脏缺血再灌注损伤的保护作用， 化疗诱导的造血干细胞毒性。核心提供了一个综合框架， 评估各个项目需求的共同成果。 目标1。在项目负责人的指导下设计、计划和监控动物实验程序。研究是 设计为达到足够的功率/灵敏度，以满足规定的目标。所有实验程序 标准化并由核心B进行，以确保所有评估的一致性。 项目这些包括：A）长寿，B）健康（代谢/认知和细胞/分子），C）压力 抗性和机制研究，D）组织收集/分布。 目标2.协调动物在项目中的使用。通过核心B获得或生成的所有动物将圈养在 南加州大学雷·R·伊拉尼大厅的最先进的动物园设施。个体专用动物饲养室 无论是育种、长寿还是抗逆的专用，都将确保生物学的一致性。 环境、噪音控制和安全。

Abstract The Animal and Biostatistics Core will work directly with Project Leaders to design, plan, monitor and interpret all animal experiments, as well as, provide specific mouse models and standardized experimental protocols in support of the overall program's common goals: 1) understand how age and genetic background modulate the effects of fasting-mimicking diets (FMD) and protein restriction cycles (PRC) on longevity and healthspan, 2) study the mechanisms of FMD-, PRC-dependent cellular protection, regeneration and rejuvenation in the hematopoietic and nervous systems, 3) understand the link between FMD and PRC, GH-IGF-1, mTOR and humanin to test the hypothesis that this mitochondrial peptide is a PFC/PRC mediator with the potential to serve as a fasting/protein restriction mimetic, 4) study the regulation of the cysteine gamma lyase-dependent production of the gas H2S, negatively regulated by GH/IGF-1-mTOR, and test the hypothesis that H2S is a mediator of the protective effects of FMD on resistance to hepatic ischemia reperfusion injury and chemotherapy-induced toxicity to hematopoietic stem cells. The core provides an integrative framework for assessment of common outcomes across the individual projects needs. Aim 1. Design, plan and monitor animal experimental procedures as directed by Project Leaders. Studies are designed to achieve sufficient power/sensitivity to meet the stated objectives. All experimental procedures performed on mice are standardized and performed by Core B to ensure consistent assessment across all projects. These include: A) Longevity, B) Healthspan (Metabolic/Cognitive and Cellular/Molecular), C) Stress resistance and Mechanistic studies, D) Tissue collection/distribution. Aim 2. Coordinate animal use for program. All animals obtained or generated through Core B will be housed in the state-of-the-art vivarium facility in the Ray R Irani Hall at USC. Individual dedicated animal holding rooms for the exclusive use of either breeding, longevity or stress resistance will ensure consistency of biological environment, noise control and security across all studies.