Vet-LIRN Network Capacity-Building Project Molecular Gel Imaging system at UI VDL

项目摘要 伊利诺伊大学(UI)兽医诊断实验室(VDL)是一项全面服务，AAVLD认证，所有 物种，参考兽医诊断实验室。作为VET-LIRN的成员，UI VDL是其中一个实验室 积极参与VET-LIRN的几项活动，包括作为牵头实验室的ISEQ-测序李斯特菌项目， 为MiSeq WGS测序项目提供分离物作为来源实验室，参与抗菌素耐药性 试点项目，以及作为牵头实验室的自动核酸提取方法的评估。凝胶图像的质量 对于传统的分子PCR来说，在对结果进行预测方面是至关重要的，而我们目前的凝胶成像系统是 非常旧，因此产生的图像质量较低。在这份提案中，我们申请资金购买Bio-Rad GelDoc Go凝胶成像系统，以提高检测动物疾病的UI VDL分子服务质量，以及 VET-LIRN协调的常规案件调查和监测。

Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference veterinary diagnostic laboratory. As a member of the VET-LIRN, UI VDL is one of the labs actively involved in several VET-LIRN activities including iSeq-sequencing Listeria project as a lead lab, providing isolates for MiSeq WGS sequencing project as a source lab, participation on antimicrobial resistance pilot project, and evaluation of automatic nucleic acid extraction methods as a lead lab. The quality of gel images for conventional molecular PCRs are critical in making call on the results and our current gel imaging system is very old, thus producing low quality of images. In this proposal, we request funding to purchase a Bio-Rad GelDoc Go Gel Imaging System to enhance UI VDL molecular service quality for testing animal diseases, and routine case investigation and surveillance coordinated by Vet-LIRN.