Vet-LIRN Network Capacity Building for SARS-CoV-2: equipment for analysis of NGS library and data

项目摘要 伊利诺伊大学（UI）兽医诊断实验室（VDL）是一个全方位的服务，AAVLD认证，所有 物种参考实验室。UI VDL是VET-LIRN的成员。截至2023年2月17日，UI VDL已测试 3，287份SARS-CoV-2样本和13个不同动物园的350份动物园动物样本检测呈阳性。下一篇： 代测序（NGS）在SARS-CoV-2的兽医诊断中对病例调查非常有用 测序方法的选择、测序文库的建立、测序结果的选择等因素对测序结果的影响 质量、病毒基因组数量、样品质量、样品合并和生物信息学分析。新设备 分析NGS库和大数据将显著提高我们的SARS-CoV-2检测能力， Vet-LIRN协调的常规病例诊断和监测的服务质量，从而增强Vet-LIRN 网络实验室进行SARS-CoV-2检测。

Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference laboratory. UI VDL is a member of the VET-LIRN. As of Feb 17, 2023, UI VDL has tested 3,287 samples for SARS-CoV-2 and 350 samples of zoo animals in 13 different zoos were tested positive. Next- generation sequencing (NGS) is very useful in case investigation of SARS-CoV-2 in the veterinary diagnostic laboratory and could be affected by many factors including selection of sequencing methods, sequencing library quality, amount of viral genome, sample quality, sample pooling, and bioinformatics analysis. New equipment for analyzing both NGS library and big data will significantly increase our SARS-CoV-2 testing capacity and service quality for routine case diagnosis and surveillance coordinated by Vet-LIRN, thus enhancing Vet-LIRN network laboratory for SARS-CoV-2 testing.