in situ Epigenetic Profiling of Single Cells in Kidney - Kim Diversity Supplement 2023

项目摘要/摘要 我们最近开发了一种基于显微镜的方法，首次能够对组蛋白进行讯问 在单个细胞中的单个基因座的修饰。这种方法被称为Sceptre(用于POST的单细胞评估 翻译表观遗传编码)，并利用超分辨率成像来直接解析和量化组蛋白 免疫荧光和DNA荧光原位标记的特定DNA或基因位点的修饰 杂交(FISH)。我们建议开发Sceptre，这是一种目前用于培养或 分离的细胞，用于肾脏组织的表观遗传学图谱，并使用Sceptre研究健康衰老和 小鼠肾组织的衰老。

Project Summary/Abstract We have recently developed a microscopy-based method that, for the first time, enables interrogation of histone modifications at single loci in single cells. This method is termed SCEPTRE (for Single-Cell Evaluation of Post TRanslational Epigenetic encoding) and utilizes super-resolution imaging to directly resolve and quantify histone modifications at specific DNA or gene loci that are labeled by immunofluorescence and DNA fluorescence in situ hybridization (FISH), respectively. We propose to develop SCEPTRE, a method currently in use with cultured or isolated cells, for epigenetic profiling of kidney tissues and to use SCEPTRE to study healthy aging and senescence in mouse kidney tissues.