PROLACTIN AND PROLACTIN RECEPTORS IN T CELL DEVELOPMENT

描述：以前的研究表明，催乳素（PRL）可能有助于 来调节T细胞的发育 这些观察结果表明，PRL PRL-R mRNA由胸腺、T细胞和胸腺上皮细胞表达， 而PRL的缺乏似乎增加了未成熟胸腺细胞的百分比。 也有相关或暗示性的证据，如 显示PRL-R表达与造血细胞 干细胞从胎儿肝脏迁移到初级淋巴器官。 最后，缺乏PRL的艾姆斯侏儒鼠表现出多种 免疫缺陷可以通过PRL治疗逆转。 这项建议 试图确定什么样的作用，如果有的话，催乳素在T细胞 发展 首先，来自胎肝的细胞表达PRL-R， 评价在不同发育阶段回收的胸腺细胞。 将通过以下方法研究艾姆斯侏儒小鼠中T细胞的成熟 T细胞表面成熟标志物的表达。 最后，胸腺细胞 将检查体外暴露于PRL的小鼠的末端脱氧核苷酸 通过RT-PCR检测转移酶表达。

DESCRIPTION: Previous studies have suggested that prolactin (PRL) may help to regulate T cell development. These observations include showing that PRL and PRL-R mRNA are expressed by thymus, T cells and thymic epithelial cells, and the lack of PRL seems to increase the percentage of immature thymocytes. There also exists a body of correlative or suggestive evidence, such as showing that PRL-R expression coincides with the time that hematopoietic stem cells migrate from the fetal liver to primary lymphoid organs. Finally, the Ames dwarf mouse, which is deficient in PRL, exhibits a variety of immunodeficiencies that can be reversed by PRL therapy. This proposal seeks to determine what role, if any, that PRL plays during T cell development. First, expression of PRL-R by cells from fetal liver and thymocytes recovered at various stages of development will be evaluated. The maturation of T cells in Ames dwarf mice will be studied by following expression of T cell surface maturation markers. Finally, thymocytes exposed to PRL in vitro will be examined for terminal deoxynucleotidyl transferase expression by RT-PCR.