CORE-- IMAGE ANALYSIS AND MORPHOMETRY

图像分析和定量形态测定核心为项目研究者提供了多种选择，用于组织学标本的定量分析。核心资源有：的Zeiss LSM（激光扫描显微镜）共聚焦显微镜;几个研究显微镜接口的摄影，数字图像捕捉和计算机驱动的阶段控制;图像数字化从显微镜照片和图纸;光盘写入档案存储;最新的生成，特殊用途和本地修改的商业和公共领域的软件;和网络连接到所有调查人员的资源评估：区域面积和卷;投射（须状）模式;神经元数目/表型;相对信息和蛋白质密度;细胞死亡;以及突起长度、分支和靶大小。由核心组织形态学专家（必要时对实验条件设盲）直接评价材料和图像，并在与核心组织统计学家协商后通过适当的统计学进行分析。核心贡献智力准备材料的介绍和出版，并使插图手稿和35毫米方。核心计划对其一些专用硬件和软件进行现代化改造。核心通过编写宏来简化数据收集。核心将通过其网页促进计划项目交流。该核心有助于该计划项目的总主题，该项目旨在了解发育可塑性的机制，作为影响神经系统的合理治疗的基础，特别是当它们与口面感觉有关时。

The Image Analysis and Quantitative Morphometry Core provides Program Project Investigators a wide variety of options for the quantitative analysis from histological preparations. The Core Resource has: a Zeiss LSM (laser scanning microscope) confocal microscope; several research microscopes interfaced for photography, digital image capture and computer driven stage control; image digitization from microscopes photographs and drawings; CD writing for archival storage; up to date generate , special purpose and locally modified commercial and public domain software; and network connections to all investigators The resources evaluates: regional areas and volumes; projection (whisker like) patterns; neuron number/phenotype; relative message and protein density; cell death; and process length, branching and target size. Materials and images are evaluated directly by expert Core morphologists (blinded to experimental conditions when necessary) and analyzed by appropriate statistics in consultation with the Core statistician. The Core contributes intellectually in preparation of materials for presentation and publication and makes illustrations for manuscripts and 35 mm sides. The Core plans to modernize some of its special purpose hardware and software. The Core streamlines data collection by writing macros. The Core will facilitate Program Project communication via its web page. This Core contributes to the general theme of this Program Project which is directed to understanding mechanisms of developmental plasticity as a basis for rational therapy of conditions affecting the nervous system, especially as they relate to orofacial sensation.