Genetics Of Obesity And Energy Metabolism

该项目的主要目的是确定人类肥胖的遗传和/或分子原因。我们使用基因测序技术来鉴定肥胖候选基因的多态性。我们使用人类神经生物学微阵列来研究肥胖和消瘦成年供体死后脑样本中的差异基因表达。在过去一年，我们有：a）继续鉴定脂肪酸合成酶基因（FAS）中的SNPs; B）继续与其他机构合作，以维持专门用于来自肥胖供体的脑样本的脑库; c）完成5名肥胖和5名瘦供体的下丘脑中的基因表达实验; d）继续进行探索性研究，使用蛋白质组学鉴定皮马人和高加索人CSF中差异分泌的蛋白质

The main aim of this project is to identify the genetic and/or molecular cause(s) of obesity in humans. We use gene sequencing techniques to identify polymorphisms in candidate genes for obesity. We use a human neurobiology microarray to study the differential gene expression in post-mortem brain samples from obese and lean adult donors. In the past year we have: a) continued the identification of SNPs in the fatty acid synthase gene (FAS); b) continued a collaborative effort with other institutions to maintain a brain bank specialized in brain samples from obese donors; c) completed gene expression experiments in the hypothalami of 5 obese and 5 lean donors; d) continued an exploratory study to use proteomics for the identification of proteins differentially secreted in the CSF of Pimas and Caucasians