Histopathology and Microscopy Core

组织学和显微镜核心（V. Lindner，核心总监） 目前的组织学和显微镜核心是在2004年在COBRE中建立的， 祖细胞生物学（8P20GM103465），与Dr. Lindner作为核心主任。服务包括 用于冷冻和石蜡包埋切片和光谱的标本的固定、包埋和处理 常规和专业组织学染色和基本免疫组化。我们的员工有专业知识， 并优化免疫组织化学程序，包括抗原修复，优化存档组织， 和多种检测方法。这一核心的成功吸引了来自我们血管姐妹公司COBRE的用户 生物学、缅因州医学中心的其他研究人员和区域客户，包括来自邻近的IDEA的客户 states.我们的服务需求量很大，并于2011年推出了血管生物学COBRE（P30GM103392） 共同资助组织学核心，以增加研究人员，扩大能力，并增加组织 微阵列服务。这一共同供资机制极为有效，得以有效扩展 服务。核心小组及其现有工作人员（核心主任和两名全职工作人员） 在过去的14年里，每年为近200种出版物提供直接支持。期间 这COBRE在代谢网络的间充质和神经调节，塑料包埋和 建立切片以及组织形态计量学以满足COBRE项目的需求。提高 效率，矿化组织组织学服务现已与 COBRE支持附近新英格兰大学的组织学和成像核心。效率和 在第一阶段，通过购置一台使用溶剂的大型组织处理机， 更有效地，从而降低成本和有害废物。作为COBRE第二阶段的一部分， 应用，我们建议整合显微镜和共聚焦显微镜服务的董事会下，博士。 Prudovsky以满足第二阶段支持的项目和其他联邦资助的研究的需要。目标1 核心是继续提供出色的服务和培训，提供全套的组织病理学服务。目的2 是将显微镜和共聚焦显微镜服务和培训无缝集成到我们的工作流程中， 更好地满足我们的COBRE调查人员和这一共享资源的其他用户的需求。Cobre 调查人员将免费获得这些服务，其他调查人员享有极具竞争力的价格。 与过去一样，核心还将继续提供广泛和不断增长的服务范围， 组织学，充分利用从以前的COBRE获得的现有设备和仪器 支持，如NIGMS所鼓励的那样。

Histopathology and Microscopy Core (V. Lindner, Core Director) The current Histopathology and Microscopy Core was established in 2004 within the COBRE in Stem and Progenitor Cell Biology (8P20GM103465), with Dr. Volkhard Lindner as the Core Director. Services included fixation, embedding, and processing of specimens for frozen and paraffin embedded sections and a spectrum of routine and specialty histological stains and basic immunohistochemistry. Our staff has expertise to develop and optimize immunohistochemistry procedures, including antigen retrieval, optimization in archival tissues, and various detection methods. The success of this Core attracted users from our sister COBRE in Vascular Biology, other investigators at Maine Medical Center, and regional clients, including from neighboring IDeA states. Our services were in high demand, and led in 2011 to our COBRE in Vascular Biology (P30GM103392) co-funding the Histopathology Core to increase research staff for expanded capacity, and add tissue microarray services. This co-funding mechanism has been extremely effective, allowing for efficient expansion of services. The Core with its current staff (Core Director and two full-time staff members) performs more than 30,000 procedures annually and in the past 14 years has directly supported close to 200 publications. During Phase I of this COBRE in Mesenchymal and Neural Regulation of Metabolic Networks, plastic embedding and sectioning as well as histomorphometry were established to meet demands by COBRE projects. To improve efficiency, mineralized tissue histology services have now been consolidated with services provided by the COBRE supported Histology and Imaging Core at the nearby University of New England. Efficiency and capacities were also increased during Phase I by acquisition of a larger tissue processor that uses solvents much more efficiently, thereby reducing costs and hazardous waste. As part of Phase II of this COBRE application, we propose to integrate microscopy and confocal microscopy services under the directorship of Dr. Prudovsky to meet the needs of Phase II supported projects and other federally funded research. Aim 1 of this core is to continue to provide outstanding services and training in a full suite of histopathology services. Aim 2 is to seamlessly integrate microscopy and confocal microscopy services and training into our workflow, to better serve the needs of our COBRE investigators and other users of this shared resource. COBRE investigators will receive these services at no charge, and other investigators enjoy highly competitive pricing. As in the past, the Core will also continue to offer its extensive and growing spectrum of services related to histology with full utilization of existing equipment and instrumentation garnered from previous COBRE support, as encouraged by NIGMS.