Biological Imaging Module

生物成像(BI)模块 摘要 生物成像(BI)模块提供最先进的设备和资源 生物组织的组织学分析和分析成像，用于训练视力研究 研究人员使用这些技术，并促进新的合作研究 既有老牌的也有新兴的调查人员。具体目标是：1)提供最先进的 眼和神经组织的分析成像的超分辨率技术，这是 超出了各个实验室的能力范围。2)为新的调查人员和工作人员提供关于 超分辨率半自动共聚焦显微镜中的先进成像技术， 反卷积和3D成像软件等模块设备和数字图形 制作。3)促进早期调查人员的研究，促进互动和 大学范围内、多元文化、不同视野的研究社区之间的合作。这个 BI模块在上一个赠款周期中得到了广泛使用，对促进 创新的视力研究，包括测试新疗法的疗效，开发和 验证用于基因治疗项目的病毒表达载体，研究受体-配体 疾病过程中的相互作用，识别生物标记物和验证蛋白质组学/代谢组学 数据，如下所述。BI模块设备在去年进行了扩展，包括新的 共聚焦显微镜系统、实时视网膜成像和敏感蛋白质分析。BI模块 已被用来生成数据，这些数据在许多出版物中都有报道，并包含在 新的助学金和R01申请，来自现有的和最近聘用的教员。该模块 还帮助招聘了一名新的教员(范博士)，他将大量使用 共聚焦实时成像系统。本模块将继续为以下方面提供基本资源 麦克奈特视力研究项目研究人员的翻译和基础科学研究 中心(MVRC)。

Biological Imaging (BI) Module Summary The Biological Imaging (BI) Module provides state-of-the-art equipment and resources for histological analysis and analytical imaging of biological tissues, for training vision research investigators in the use of these technologies, and to promote new collaborative studies among established and emerging investigators. Specific Aims are: 1) To provide state-of-the-art super-resolution technology for analytical imaging of ocular and neural tissues, which are beyond reach of individual laboratories. 2) To provide training for new investigators and staff on advanced imaging techniques in super-resolution semi-automated confocal microscopy, deconvolution and 3D imaging software and other module equipment and digital graphics production. 3) To promote research of early stage investigators and foster interactions and collaborations among a university-wide, multi-cultural, diverse vision research community. The BI module was extensively used in the last grant cycle and was essential for promoting innovative vision research, including testing efficacy of novel therapies, developing and validating viral expression vectors for gene therapy projects, investigating receptor-ligand interactions during disease, identifying biomarkers and validating proteomics/metabolomics data, as described below. BI module equipment was expanded in the last year to include new confocal microscopy systems, live retinal imaging and sensitive protein analysis. The BI module has been used to generate data that was reported in numerous publications and included in new grant awards and R01 applications from established and recently hired faculty. The module also facilitated recruitment of a new faculty member (Dr. Pham) who will be a heavy user of the confocal live imaging system. This module will continue to provide an essential resource for translational and basic science research for investigators of the McKnight Vision Research center (MVRC).