Core Grant for Vision Research

总结 我们的视觉研究核心的总体目标是提供专业知识，设施和设备，以促进和 在威斯康星大学麦迪逊分校扩展视觉研究。这将由三个核心来完成。 眼科组学和定量分子生物学核心将促进qPCR、激光捕获 显微切割、RNASeq、微阵列、NextGen测序、流式细胞术和其他分子方法 核心用户。病理学和成像核心将有助于使用共聚焦显微镜，荧光和 光学显微镜、组织病理学、专门的组织染色方法和核心用户的图像分析。的 动物模型和眼器官培养核心将促进非人灵长类动物、猫科动物、啮齿类动物和 其他眼部疾病的动物模型，OCT、ERG、VEP评估视功能的用途，以及 其他视觉结构和功能结果测量。

SUMMARY The overall goal of our Vision Research Core is to provide expertise, facilities, and equipment to foster and expand vision research at the University of Wisconsin-Madison. This will be accomplished by three Cores. The Ocular Omics and Quantitative Molecular Biology Core will facilitate the use of qPCR, Laser Capture Microdissection, RNASeq, microarrays, NextGen Sequencing, Flow Cytometry and other molecular methods by core users. The Pathology and Imaging Core will facilitate the use of confocal microscopy, fluorescent and light microscopy, histopathology, specialized tissue staining methods, and image analysis by core users. The Animal Models and Eye Organ Culture Core will facilitate the use of non-human primate, feline, rodent and other animal models of ocular disease, the use of OCT, ERG, VEP to assess visual function, and the use of other visual structure and function outcome measures.