BASAL HEPATIC GLUCOSE AND INSULIN SENSITIVITY IN BABOONS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是通过在狒狒中进行正常血糖-高胰岛素钳夹程序来评估葡萄糖代谢，以比较肥胖、消瘦和糖尿病动物之间的葡萄糖清除率差异。 正常血糖-高胰岛素钳夹程序被认为是测量胰岛素敏感性的“金标准”技术，并且在人类受试者中常规进行，并且将允许我们评估这些动物中的胰岛素敏感性。 在正常血糖-高胰岛素钳夹过程中，循环葡萄糖通过输注外源性葡萄糖来保持恒定，以响应胰岛素水平的升高。 作为该项目的一部分，我们还收集了肌肉，脂肪和肝脏活检，同时在胰岛素管理，以检查组织特异性差异的葡萄糖利用，胰岛素信号和基因表达模式。我们的主要目标是验证该协议的长期研究中的胰岛素-葡萄糖轴的分子遗传学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study was to conduct assessments of glucose metabolism by performing the euglycemic-hyperinsulinemic clamp procedure in baboons in order to compare the difference in glucose clearance between obese, lean, and diabetic animals. The euglycemic- hyperinsulinemic clamp procedure is considered the "Gold Standard" technique to measure insulin sensitivity, and is routinely performed in human subjects, and will allow us to evaluate insulin sensitivity in these animals. During the euglycemic-hyperinsulinemic clamp procedure circulating glucose is held constant by infusion of exogenous glucose in response to rising insulin levels. As part of this project we have also collected muscle, fat and liver biopsies while under insulin administration to examine tissue-specific differences in glucose utilization, insulin signaling, and gene expression patterns. Our major objective is to validate this protocol for long term studies in molecular genetics of the insulin-glucose axis.