Role of estrogen and its receptors in ovarian cancer of the hen

项目总结： 卵巢癌是女性癌症死亡的第五大原因。众所周知，有一种 接受雌激素替代治疗的女性患卵巢癌的风险增加 服用避孕药的女性风险可能降低，这支持了类固醇 荷尔蒙在卵巢癌的病因中起着作用。该项目旨在阐明 家母鸡卵巢癌中雌激素及其受体的研究。这只母鸡是个好榜样，因为它 患上卵巢癌，自发性高。本研究的具体目的是：1) 确定母鸡的卵巢癌是否依赖雌激素；2)确定雌激素受体是否 在卵巢癌中的差异调控；3)非核雌激素受体的特征；以及4) 确定与雌激素受体介导的增殖相关的下游通路。这些 问题将使用各种分子技术来回答，包括聚合酶链式反应、蛋白质印迹、 免疫组织化学和微阵列分析，体外方法包括细胞培养、体外(或体内) 方法包括正常和肿瘤卵巢组织的CAM移植，以及包括治疗在内的体内方法 母鸡的雌二醇量。一旦这些问题得到回答，我们将对这一角色有更好的理解 卵巢癌中雌激素及其受体的研究。这些答案还将使我们了解 雌激素促进母鸡卵巢肿瘤形成或增殖的机制 可能与雌激素及其受体在人卵巢癌中的作用有关。此外， 了解雌激素的作用可能会导致可能适用于人类的治疗方案 疾病。 相关性： 卵巢癌是女性癌症死亡的第五大原因。雌激素可能促进 卵巢肿瘤的起始或增殖；因此，重要的是要了解它在起源或增殖中的作用。 疾病的发展。

Project summary: Ovarian cancer is the fifth leading cause of cancer death among women. It is known that there is an increased risk of ovarian cancer in women who are on estrogen-only hormone replacement therapy and possible decreased risk in women who take the contraceptive pill, lending support to the idea that steroid hormones are playing a role in the etiology of ovarian cancer. This project is designed to clarify the role of estrogen and its receptors in ovarian cancer of the domestic hen. The hen is a good model because it develops ovarian cancer with a high spontaneous incidence. The specific aims of this study are to: 1) determine whether ovarian cancer in the hen is estrogen dependent; 2) determine if the estrogen receptor is differentially regulated in ovarian cancer; 3) characterize the non-nuclear estrogen receptor; and 4) determine downstream pathways associated with estrogen receptor mediated proliferation. These questions will be answered using a variety of molecular techniques including PCR, Western blot, immunohistochemistry and microarray analyses, in vitro methods including cell culture, ex vivo (or in ovo) methods including CAM grafts of normal and tumor ovarian tissue, and in vivo methods including treatment of hens with estradiol. Once these questions are answered, we will have a better understanding of the role of estrogen and its receptors in ovarian cancer. These answers will also allow us to understand the mechanism of estrogen action promoting the formation or proliferation of ovarian tumors in the hen, which may be correlated to the role estrogen and its receptors m,ay play in human ovarian cancer. Furthermore, understanding estrogen's actions might lead to possible treatment options that could be applicable to human disease. Relevance: Ovarian cancer is the fifth leading cause of cancer death among women. Estrogens may promote ovarian tumor initiation or proliferation; therefore, it is important to understand its role in the origin or progression of the disease.