MICROSPHERE BASED DNA ANALYSIS FOR PATHOGEN POINT DETECTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们将扩大目前在开发微球分析方面的努力，以开发一种新的环境样本遗传分析方法，从而能够以高度的置信度和低误报率灵敏、快速地检测病原体。 具体而言，我们会发展多重微球检测试剂盒，以提供快速点检测，检测从环境样品扩增的病原体特异性PCR产物，从而在单一测试中筛查一组微生物。 由于流式细胞术是分析平台，样品制备简单，不需要洗涤步骤，分析快速，不到一分钟。 新的检测方法将与商业实验室流式细胞仪以及较小的现场仪器兼容，包括作为DOE CBNP工作的一部分正在开发的自主病原体检测器（APD）。 这些新工具将允许灵活设计，以便在各种应对方案中提供快速分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will expand our ongoing efforts in the development of microsphere-based assays to develop a new approach to the genetic analysis of environmental samples that will enable sensitive and rapid detection of pathogens with a high degree of confidence and a low false alarm rate. Specifically, we will develop multiplex microsphere-based assay kits to provide rapid point detection of pathogen-specific PCR products amplified from environmental samples, allowing screening for a panel of organisms in a single test. Because flow cytometry is the analysis platform, sample preparation is simple, requiring no wash steps, and analysis is rapid, less than one minute. The new assays will be compatible with commercial laboratory flow cytometers as well as smaller fieldable instruments, including the Autonomous Pathogen Detector (APD) being developed as part of the DOE CBNP effort. These new tools will allow flexible design to provide rapid analysis in a variety of response scenarios.