IMPROVED PERFORMANCE OF 4-PI MICROSCOPY USING HILBERT PHASE MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 4-Pi显微镜已成为一种强大的高分辨率细胞和组织成像技术。然而，这些结构中存在的折射率不均匀性限制了其性能。我们的初步研究表明，我们的希尔伯特相位显微镜可以提供有关样品的折射率信息。这将有助于改善组织折射率匹配，从而改善整体4-Pi显微镜性能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 4-Pi microscopy has become a powerful high-resolution technique for imaging cells and tissues. However, refractive index inhomogeneities present in these structures limit its performance. Our preliminary studies show that, our Hilbert phase microscopy can provide the refractive index information about the sample. This will help improve the tissue index matching and, thus, the overall 4-Pi microscopy performance.