TOMOGRAPHIC PHASE MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们已经开发出一种技术，用于定量三维映射的折射率在活的生物细胞和组织。样品折射率的投影成像使用马赫-曾德尔移相外差干涉仪与多个方向的照明。然后，使用滤波反投影和迭代约束算法从2-D投影相位图像的集合导出3-D层析重建。折射率图将在高分辨率显微镜、细胞光散射和用于疾病诊断和成像的光学方法中具有应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a technique for quantitative three-dimensional mapping of refractive index in live biological cells and tissues. The projection of sample refractive index is imaged using a Mach-Zehnder phase-shifting heterodyne interferometer with multiple directions of illumination. A 3-D tomographic reconstruction is then derived from the collection of 2-D projection phase images using a filtered back-projection and iterative constraint algorithm. Refractive index maps will have applications in high resolution microscopy, cellular light scattering, and optical methods for disease diagnosis and imaging.