MULTIMODAL SPECTROSCOPY PROBE DEVELOPMENT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 我们正在研究将反射率和荧光的诊断信息与拉曼光谱法获得的信息相结合的实用性  称为多模态光谱（MMS）-诊断动脉粥样硬化和乳腺癌。因此，正在开发一种单一的光谱探头，用于所有三种模式。组合探针将从现有临床FastEEM和拉曼源获取激发光，并将光传递到相同的组织位置并从相同的组织位置收集光。组合探头设计为能够提供和收集300-1000 nm光谱范围内的光。基于已成功用于外科手术室的体内拉曼探针，组合探针将使多个源耦合到其单个激发光纤中，将激发和发射滤波器与专门设计的光学涂层结合以抑制光纤背景并使所需信号的有效提取成为可能。收集光纤将被分开，并被引导到两个光谱仪/CCD模块进行光谱分析和参数提取。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the utility of combining diagnostic information from reflectance and fluorescence to that obtained by Raman spectroscopy  termed multimodal spectroscopy (MMS) - to diagnose atherosclerosis and breast cancer. As a result, a single spectral probe is being developed to be used with all three modalities. The combined probe would take excitation light from existing clinical FastEEM and Raman sources and deliver to and collect the light from the same tissue location. The combined probe is designed to be capable of delivering and collecting light in the 300-1000 nm spectral range. Based on the in vivo Raman probe that has been successful used in the surgical theatre, the combined probe will enable the coupling of multiple sources into its single excitation fiber, incorporate excitation and emission filters with specially designed optical coatings to suppress fiber background and enable efficient extraction of the desired signal. Collection fibers would be split up and be directed to two spectrograph/CCD modules for spectral analysis and parameter extraction.