FIBER WAX COMMISSIONING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 去年加强的一项活动是WAXS的开发 纤维结晶学 与Stubbs集团（范德比尔特大学）和Orgel集团（以前的罗莎琳德富兰克林大学，现在在IIT）合作驱动的能力。 波荡器辐射用于这种研究的好处是能够将其准直到样品处相对较小的光束尺寸（60 x 60 <$m2），以照射样品中的小有序域，同时具有足够低的发散度以解析图案中的精细细节。为了进行这些实验，我们需要从SAXS装置中拆卸各种部件，并使用从Stubbs小组借来的飞行管来组装工作仪器。 我们现在拥有设计下一个融资期内将建立的专用设置所需的知识。 除了具有专用组件的专用设置外，还确定了所需的增强功能：高分辨率实时样品对准光学器件、样品测角和低温功能。此外，还发现需要进行生物安全2或3实验。BSL 2实验之前已经在BioCAT运行过，但在程序上执行起来并不简单，而BSL 3实验根本不可能在18 ID运行。然而，我们最近成功地证明了在BioCARS 14 BM-C上进行某些技术要求较低的纤维衍射实验的可行性。 目前使用的是每次运行约4天。我们已经与BioCARS达成协议，也是NCRR生物技术研究资源，以支持他未来的活动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An activity that has intensified in the last year is the development of WAXS Fiber Crystallography capabilities driven by collaborations with the Stubbs group (Vanderbilt University) and Orgel group (formally Rosalind Franklin University, now at IIT). The benefit of undulator radiation for this kind of study is the ability to collimate it to relatively small beam sizes at the sample (60 x 60 ¿m2) to illuminate small ordered domains in samples while having low enough divergence to resolve fine detail in the patterns. In order to perform these experiments, we have needed to cannibalize various components from the SAXS setup and use flight tubes borrowed from the Stubbs group to assemble a working instrument. We now have the knowledge needed to design a dedicated setup to be built in next funding period. In addition to having a dedicated setup with purpose built components, needed enhancements have been identified: high-resolution real-time sample alignment optics, sample goniometry and cryogenic capabilities. In addition, it has been found that there is a need to perform Biosaftey 2 or 3 experiments. BSL2 experiments have been run at BioCAT before, but are procedurally non-trivial to execute and BSL3 experiments are not possible at 18ID at all. However, we have recently successfully demonstrated the feasibility of performing certain less technically demanding fiber diffraction experiments at BioCARS 14 BM-C. Present use is about 4 days per run. We have come to an agreement with BioCARS, also a NCRR Biotechnology Research Resource, to support his activity in the future.