HISTONE METHYLTRANSFERASE COMPLEX AND EPIGENETIC CONTROL OF THE GENOME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 组蛋白的残基特异性甲基化导致不同的表观遗传状态。但是，组蛋白甲基转移酶(将甲基添加到组蛋白中的酶)是如何针对特定的染色体区域的还不是很清楚。更重要的是，除了几个众所周知的例子外，细胞机制如何识别这些修改还不清楚。我们之前对H3K9甲基转移酶的纯化揭示了蛋白质泛素化和RNAi介导的异染色质组装之间令人惊讶的联系。我计划提纯组蛋白甲基转移酶复合体，以确定调节组蛋白甲基化的新蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Residue specific methylation of histones results in diverse epigenetic states. But how histone methyltransferase (enzymes that add methyl group to histones) are targeted to specific chromosome domains are not well understood. More importantly, apart from several well-known examples, how the cellular machinery recognizes these modifications is not clear. Our previous purification of H3K9 methyltransferase uncovered a surprising link between protein ubiquitylation and RNAi mediated heterochromatin assembly. I plan to purify histone methyltransferase complexes to identify novel proteins that regulate histone methylation.