NMSU CELL AND ORGANISM CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 新墨西哥州州立大学的细胞和有机体核心设施(COCF)提供了一个适当的设施，配备了仪器，用于利用新墨西哥州Brin/INBRE赠款的资金进行组织培养实验。对于NMSU生物和化学/生物化学系的初级教员在他们的研究中使用体外方法的研究项目，以及一些通过试点项目获得INBRE资助的初级教员来说，这个设施是一项重要的资产。因此，COCF的功能符合新墨西哥州INBRE的声明目标之一：为研究人员和学生创建一个研究基础设施，以使用原代细胞培养物、来自各种动物生物体的活组织外植体制剂和已建立的细胞系进行研究。此外，COCF支持有利于NMSU非INBRE研究人员的研究活动，并促进社区中非大学科学家的使用。具体而言，COCF目前支持有学生参与的研究活动，重点是：1)参与细胞质分裂和细胞周期调控的分子机制(Charles Shuster博士)；2)脊椎动物再生过程中的细胞去分化和转分化(Graciela Unguez博士)，3)哺乳动物胚胎干细胞中硬骨肌调节因子的功能特征及其成肌转换能力(Graciela Unguez博士)，4)Grb7及其候选结合蛋白在哺乳动物细胞中的蛋白质间相互作用(Barbara Lyons博士)。COCF正在推动日常研究活动，不仅涉及教职员工，还涉及他们的研究生和本科生。在过去的7个月里，共有13名学生(6名研究生，7名本科生)接受了细胞培养技术的培训，并制定了针对组织特异性体外研究的优化方案。该设施的进一步成熟将允许更广泛的研究和教学项目，并支持更多的NMSU研究人员和访问科学家的需求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell and Organism Core Facility (COCF) at New Mexico State University provides a proper facility equipped with instrumentation to carry out tissue culture experiments using funds from the New Mexico BRIN/INBRE grant. This facility is a critical asset to the research programs of junior faculty in the Biology and Chemistry/Biochemistry Departments at NMSU who use in vitro approaches in their studies  and some junior faculty who also receive INBRE funding through pilot projects. Hence, the function of the COCF meets one of the stated goals of the New Mexico INBRE: to create a research infrastructure for investigators and students to perform studies using primary cell cultures, live tissue explant preparations from a variety of animal organisms, and established cell lines. In addition, the COCF supports research activities that benefit non-INBRE researchers at NMSU and promotes its use by non-university scientists in the community. Specifically, the COCF currently supports research activities that involve students and focus on: 1) molecular mechanisms involved in the timing of cytokinesis and cell cycle regulation (Dr. Charles Shuster) 2) cell dedifferentiation and transdifferentiation during regeneration in vertebrates (Dr. Graciela Unguez), 3) functional characterization of teleost muscle regulatory factors in mammalian embryonic stem cells and their myogenic conversion capacity (Dr. Graciela Unguez), 4) protein-protein interaction of Grb7 and its candidate binding proteins in mammalian cells (Dr. Barbara Lyons). The COCF is enabling daily research activities involving not only faculty, but their graduate and undergraduate students as well. In the past 7 months, a total of thirteen students (6 graduate, 7 undergraduate) have been trained in cell culture techniques and have developed protocols optimized for tissue-specific in vitro studies. Further maturation of the facility will allow for a broader range of research and instructional projects and support the needs of additional NMSU researchers and visiting scientists.