Microvessel Density With High Spectral/Spatial MRI

MRI发现致密的、可渗透的血管与恶性肿瘤的血管生成有关。 对比剂的摄取。然而，还需要提高核磁共振成像的准确性。改进检测 MRI对血管生成的研究将对肿瘤的检测和治疗产生重大影响。 该实验室以前的工作表明，高光谱和空间分辨率(HISS)磁共振成像增加 对造影剂效果的敏感性，因为它检测到对比度的光谱不均匀影响 代理(CA)。单个小体素对水共振的小分量的CA效应可能 提示存在亚体素血管新生病灶。这项拟议的研究验证了这一假设 对比剂增强的HISS MRI提高了检测ANG/GENES/S的敏感性和特异性。 开发获取和处理HISS数据集的方法，以最大限度地提高对造影剂和 评估HISS MRI是否能准确检测血管生成。他的核磁共振测量了血容量， 我们将评估极小体素的毛细血管渗透性和血管构筑。嘘声的敏感度 将MRI对血管结构和功能的表现与高分辨率常规MRI进行比较。 为了开发和评估肿瘤血管的HISS检测，我们将： 1.改进获取和处理HISS数据的方法，以优化对造影剂的敏感性。 2.利用高分子量CA的HISS成像测量血管体积和通透性。 3.将HISS数据与常规高分辨率MRI方法进行比较。 4.将HISS图像数据与组织学测量的微血管密度和血管铸型进行比较。 5.检测HISS检测抗血管生成治疗对肿瘤血管的影响。 这项提案要求支持开发创新的磁共振方法，以攻击重要的 临床问题。创新之处在于：1)HISS是核磁共振成像的一种新方法2)HISS效应检测 对比剂的使用是动态增强MRI的一种新方法。HISS MR数据目前 在全身扫描仪上使用回声平面光谱成像在临床可接受的运行时间内获得 (EPSi)。因此，拟议工作的结果可以迅速转化为临床实践，并将 改善患者护理。

MRI identifies dense, permeable vasculature associated with angiogenesis in malignant tumors based on uptake of contrast agents. However, increases in the accuracy of MRI are needed. Improving detection of angiogenesis by MRI would have a significant impact on detection and treatment of cancer. Previous work in this laboratory showed that high spectral and spatial resolution (HiSS) MRI increases sensitivity to the effects of contrast agents because it detects spectrally inhomogeneous affects of contrast agents (CA's). CA effects on small components of the water resonance from single small voxels may indicate the presence of sub-voxelar angiogenic foci. The proposed research tests the hypothesis that contrast enhanced HiSS MRI has increased sensitivity and specificity for detection of ang/ogenes/s. We will develop methods for acquiring and processing HiSS datasets to maximize sensitivity to contrast media and evaluate whether HiSS MRI accurately detects angiogenesis. HiSS MRI measurements of blood volume, capillary permeability, and vascular architecture in very small voxels will be evaluated. Sensitivity of HiSS MRI to vascular structure and function will be compared to that of high-resolution conventional MRI. To develop and evaluate HiSS detection of tumor vasculature we will: 1. Improve methods for acquiring and processing HiSS data that optimize sensitivity to contrast agents. 2. Measure vascular volume and permeability using HiSS imaging of high molecular weight CA's. 3. Compare HiSS data with conventional high-resolution MRI methods. 4. Compare HiSS image data to histological measurements of micro-vessel density and vascular casts. 5. Test the ability of HiSS to detect effects of anti-angiogenic therapy on tumor vasculature. This proposal requests support for development of innovative MR methods that attack an important clinical problem. The areas of innovation are 1) HiSS is a new approach to MRI 2) HiSS detection of effects of contrast agents is a novel approach to dynamic contrast enhanced MRI. HiSS MR data are currently acquired in clinically acceptable run-times on whole-body scanners using echo-planar-spectroscopic imaging (EPSI). Thus, the results of the proposed work could be rapidly translated to clinical practice and would improve patient care.