COBRE: UID: MOLECULAR BIOLOGY CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子生物学核心设施为研究人员提供了分析核酸序列的最先进的仪器、设备和设施。核心设施现在占地850平方英尺。该实验室位于南生命科学中心，是新装修的空间的一部分，配备了下文所述的主要仪器和计算资源，以及湿实验室工作台、两个努伊尔生物安全柜、热循环仪、离心机、微熔断器、冷冻机、冰箱和样品制备所需的其他次要设备。一名全职技术人员监督仪器的操作和维护，并为设施的用户提供指导、指导和故障排除帮助。核心设施现有的主要仪器包括：两台毛细管DNA测序仪(一台应用生物系统公司的PRISM 3730 DNA分析仪，一台ABI 3730是48毛细管系统)，一台Qigen BioRobot 3000，用于使用96孔微孔板格式进行高纯度样品处理。该实验室的一部分专门用于数据分析和生物信息学。该实验室配备了两台Macintosh Power Mac G5计算机，具有双2 GHz处理器、1 GB RAM和两个250 GB硬盘，这些硬盘通过安全的高速本地网络进行联网。这为调查人员提供了访问互联网上和通过生物信息学核心设施可用的资源的机会。这些都是最先进的设施，支持爱达荷大学广泛的生物医学研究项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core Facility has provided researchers access to state-of-the-art instruments, equipment, and facilities for the analysis of nucleic acid sequences. The Core Facility now occupies 850 sq. feet of newly renovated space in Life Sciences South, and is equipped with the major instrumentation and computational resources described below, as well as wet lab benches, two NuAire biosafety cabinets, thermocyclers, centrifuges, microfuges, freezers, refrigerators, and other minor equipment needed for sample preparation. A full time technician oversees operation and maintenance of the instruments, and provides instruction, guidance, and trouble-shooting assistance to users of the facility. The major instrumentation now available in the Core Facility includes the following: two capillary DNA sequencers (an Applied Biosystems PRISM 3730 DNA Analyzer, and an ABI 3730 is a 48 capillary system), a Qiagen BioRobot 3000 for high thoroughput sample processing using a 96-well microplate format. A portion of the laboratory is dedicated to data analysis and bioinformatics. The laboratory is equipped two Macintosh Power Mac G5 computers which have dual 2 GHz processors, 1GB of RAM, and two 250 GB hard drives that are networked through a secure high-speed local network. This provides investigators with access to resources that are available on the Internet and through the Bioinformatics Core Facility. These are state of the art facilities that support a wide range of biomedical research projects at the University of Idaho.