Cell Culture and Virus Vector Core

细胞和病毒载体核心实验室的任务是提供与细胞培养相关的试剂和 满足癌症中心研究社区需求并支持高质量研究的服务。 CWCL储存媒体和试剂，并在严格的质量控制下准备定制的媒体配方 手术，为癌症中心的成员节省了相当多的费用。该委员会的其他职能包括 保存冷冻细胞系、病毒库存和病毒载体的储存库，以建立原代细胞培养，以 扩大组织培养细胞用于大规模实验，并进行支原体检测。CWCL 还通过为癌症中心研究人员提供定制的帮助来满足他们的科学需求 研究项目。自1978年成立以来，CWCL一直与 研究人员开发对其研究至关重要的工具和方法。最近的一些例子包括 方法制备用于实验性前列腺和脑的痘苗病毒和猿猴病毒5号 CCCWFU调查人员的癌症治疗。此外，通过酶联免疫吸附试验和斑块分析来评估新的 用于抗肝炎病毒和HIV-1活性的化合物，用于评估抗癌前景的细胞毒性试验 试剂，发现新癌基因的改进的焦点形成试验，以及重组人 利用逆转录病毒载体或四环素诱导载体的蛋白质已经被开发出来。现成的可用性 试剂和细胞与CVVCL员工的专业知识相结合，大大加快了开发速度 并实施新的研究项目，减少雇用和培训新人员的需要。因此， CWCL是癌症中心的重要组成部分，可以最大限度地提高研究人员的工作效率 并使他们在获得外部赠款支持方面保持竞争力。 自2001年以来，细胞和病毒媒介核心实验室的使用率以每年-20%的速度增长， 人员数量的变化。在2005财年，CVVCL 60%以上的退款收入来自 癌症中心成员，对已批准的癌症相关项目进行积极的同行评议研究。目前， 用户按存储容量使用计费占核心总运营预算的-78%，CCSG和我们机构 两者都支持--占总运营预算的11%。以满足癌症中心日益增长的需求 对于调查人员，我们预计到2007年将有必要招聘更多的人员。

The mission of the Cell and Virus Vector Core Laboratory is to provide cell culture-related reagents and services that meet the needs of the Cancer Center research community and support high-quality research. The CWCL stocks media and reagents and prepares custom media formulations under strict quality-control procedures, at considerable savings for Cancer Center members. Other functions of the CCWL are to maintain a repository of frozen cell lines, virus stocks and virus vectors, to establish primary cell cultures, to expand tissue culture cells for large-scale experiments, and to perform mycoplasma assays. The CWCL also serves the scientific needs of the Cancer Center researchers by offering customized assistance for research projects. Since its inception in 1978, the CWCL has worked in close collaboration with investigators to develop tools and methods essential for their research. Some recent examples include methods to produce vaccinia virus and simian virus 5 that are used for experimental prostate and brain cancer treatments by CCCWFU investigators. In addition, ELISA tests and plaque assays to evaluate novel compounds for anti-hepatitis virus and HIV-1 activity, cytotoxicity assays to evaluate prospective anticancer agents, modified focus formation assays to uncover novel oncogenes, and the expression of recombinant proteins using retroviral vectors or tetracycline-inducible vectors have been developed. The ready availability of reagents and cells combined with the expertise of the CVVCL staff greatly speeds up the development and implementation of new research projects and reduces the need to hire and train new personnel. Thus, the CWCL is an essential component of the Cancer Center that maximizes the productivity of investigators and enables them to stay competitive for obtaining extramural grant support. Usage of the Cell and Virus Vector Core Laboratory has increased by -20% yearly since 2001 without changes in personnel number. Over 60% of the CVVCL charge-back income in fiscal year 2005 came from Cancer Center members with active peer-reviewed research on approved cancer-related projects. Currently, user charge backs account for -78% of the core's total operating budget, and the CCSG and our Institution both support -11% of the total operating budget. To meet the growing needs of the Cancer Center investigators, we anticipate it will become necessary to hire additional personnel by year 2007.