Transgenic Knockout Mice

转基因/敲除小鼠共享资源 Kimmel癌症中心转基因/敲除小鼠共享资源协助研究人员 设计、创建、保存、维护和传播人类疾病的小鼠模型。它服务 两个功能：1）作为转基因项目战略的信息资源， 转基因和靶向载体，以及用于小鼠模型的分析和维持;以及2）作为 提供必要的技术专长和服务，以生产和维护这些模型。 共享资源提供以下技术服务：1）通过转基因小鼠的生产 研究者的DMA构建体的原核显微注射; 2）完成基因靶向服务， 研究者的靶向构建体或预先靶向的ES细胞和返回的嵌合小鼠携带 工程突变; 3）有价值的小鼠品系的冷冻保存和小鼠从冷冻中的恢复 种质;和4）一系列辅助生殖技术，可以应用于维持小鼠 以更具成本效益或时间效益的方式进行应变。

TRANSGENIC/ KNOCKOUT MOUSE SHARED RESOURCE The Kimmel Cancer Center Transgenic/ Knockout Mouse Shared Resource assists investigators in designing, creating, preserving, maintaining and disseminating mouse models of human disease. It serves two functions: 1) As an information resource for strategies for transgenic projects, for the proper design of transgenic and targeting vectors, and for the analysis and maintenance of mouse models; and 2) As a provider of the necessary technical expertise and services to produce and maintain these models. The shared resource provides the following technical services: 1) the production of transgenic mice via pronuclear microinjection of an investigator's DMA construct; 2) complete gene targeting services, taking an investigator's targeting construct or pre-targeted ES cells and returning chimeric mice carrying the engineered mutation; 3) cryopreservation of valuable mouse strains and recovery of mice from frozen germplasm; and 4) a range of assisted reproductive techniques that can be applied to maintaining mouse strains in a more cost- or time-effective manner.