ASSISTED REPRODUCTIVE TECHNIQUES IN RHESUS MACAQUES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。通过使用从特定雄性中收集的冷冻保存的精子来使适当的雌性受精，可以最大限度地提高受控繁殖的效率，以产生特定基因类型的恒河猴。但这是假设不同基因类型的男性的精液可以同样有效地冷冻保存。为了研究不同雄性猕猴的精子在相同条件下冷冻后是否能被有效地冷冻，我们收集并冷冻了来自三个灵长类研究中心的13只成年、有生育能力的雄性猕猴的精液样本。解冻后的存活率在解冻后30分钟内进行测定，存活率从50%到70%不等，但此后有所下降。为了检测个体雄性精液的反应，我们收集并冷冻了7只雄性精液中的每只精液3到6个。一般说来，给定男性的精液对冷冻的反应是可重复的，但男性之间存在显著差异。雄鱼在解冻后精子存活率方面存在这些差异的原因尚不清楚。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The efficiency of controlled propagation to produce rhesus monkeys of particular genotypes can be maximized by use of cryopreserved spermatozoa collected from specific males to inseminate appropriate females. But this assumes that semen from males with different genotypes can be cryopreserved with equal effectiveness. To investigate whether spermatozoa from different Macaca mulatta males can be effectively cryopreserved when frozen under identical conditions, we collected and froze semen specimens from 13 adult, fertile males maintained at three primate research centers. Survival, based on post-thaw motility normalized to the pre freeze value, was assayed within 30 minutes after thawing; it varied from 50 to 70% but declined thereafter. To examine the response of semen from individual males, we collected and froze three to six ejaculates/male from each of seven males. In general, semen from a given male responded reproducibly to freezing, but there were significant differences among males. The cause of these differences among M. mulatta males in post-thaw sperm survival remains unidentified.