CORE 1 TRP3: PRODUCT TERMINAL ISOTOPE CODING (PROTIC)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 技术研究项目3，产品终端同位素编码(PRIC)，将重点放在 蛋白质降解最终产物的蛋白质组学特征。识别所有产品的能力，以及 然后推断最初的底物，因为蛋白水解酶本质上是蛋白质分解的“圣杯”。这 能力将改变细胞生物学领域。这项任务的重要性与其难度相匹配。我们建议开发一种全新的化学策略用于产品末端同位素编码(PRIC)，即生物样品中所有N末端的同位素标记。这种标记方法将允许标识和相关的 复杂蛋白质组底物/产物图谱的定量研究

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technical Research Project 3, Product Terminal Isotope Coding (PROTIC), will focus on the proteomic characterization of the end products of proteolysis. The ability to identify all products, and then infer the initial substrates, for a protease is essentially the "holy grail" of proteolysis. This capability would transform the field of cell biology. The significance of the task is matched by its difficulty. We propose to develop an entirely novel chemical strategy for Product Terminal Isotope Coding (PROTIC), the isotope tagging of all N-termini in biological samples. This tagging method will allow the identification and relative quantification of substrate/product profiles in complex proteomes