Flourescent Probes & Imaging - Networks & Pathways(RMI)

我们建议在全国范围内建立一个以荧光探头和成像为重点的可见和响应中心 在活细胞中实时、高分辨率地研究通路和网络的技术。这个 拟建的中心将结合现有的两个中心的经验和基础设施 匹兹堡的中心。该中心的研究部分将创建一个强大的细胞内工具箱 荧光标记和生物传感器，可用于研究许多(如果不是全部)途径和 活细胞网络。强荧光探针开发计划融合了遗传学、蛋白质 结构、核酸结构和荧光染料化学。我们建议生成的探测器将是 基因可表达，因此外源大分子不必运输到活细胞中 有待研究。 四个极好的“驾驶生物学项目”将是整个中心计划的一部分，除了 技术开发核心。这些项目位于卡内基梅隆的匹兹堡大学， 斯坦福和伯克利。他们的职责是协助新中心确定最佳技术 用于发展，并提供关于技术效用的重要反馈。 成像和信息学技术与新的荧光探针技术相结合，从而 这些组合工具将帮助细胞生物学家获得关于 活细胞中的通路。提案中包括制造荧光探针和成像的程序 强大的技术，以便它们可以在学术研究中提供给广泛的细胞生物学家， 药物发现和生物技术，并传播这些技术和提供 训练。 性能

We propose to form a nationally visible and responsive center focused on fluorescent probe and imaging technologies for investigating pathways and networks in real time and at high resolution in living cells. The proposed Center will be formed by combining the experience and infrastructure of two already existing Centers in Pittsburgh. The research component of the Center will create a powerful toolbox of intracellular fluorescent labels and biosensors that can be used to study many, if not all, the proteins in pathways and networks of living cells. The strong fluorescent probe development program blends genetics, protein structure, nucleic acid structure and fluorescent dye chemistry. The probes we propose to generate will be genetically expressible so that exogenous macromolecules will not have to be transported into the living cells to be studied. Four superb "Driving Biology Projects" are to be part of the overall Center program in addition to the Technology Development Core. These projects are located at the University of Pittsburgh, Carnegie Mellon, Stanford and Berkeley. Their responsibility is to assist the new center in identifying the optimal technologies for development and for providing crucial feedback regarding the utility of the technologies. Imaging and informatics technologies are integrated with the new fluorescent probe technologies so that these combined tools will help cell biologists obtain large amounts of spatial and temporal information about pathways in living cells. Included in the proposal are programs for making the fluorescent probe and imaging technologies robust so that they can be provided to a wide range of cell biologists in academic research, pharmaceutical drug discovery, and biotechnology and to disseminate these technologies and provide training. PERFORMANCE