DEVELOPMENT OF BIOMARKERS TO ASSESS COMMUNITY LEVEL EXPOSURE TO TOXICANTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 生物标记物是可以在血液、尿液或其他生物标本中检测到的生物变化。它们可以在暴露时和在临床疾病出现之前很久就提供接触有毒物质的迹象。有必要确定足够敏感的生物标志物，以检测社区中发现的低水平污染物化学物质的暴露。本研究的目的是调查检测环境污染物暴露的炎性和遗传毒性反应的生物标志物的敏感性。我们假设，居住在有毒空气污染物主要点源附近的社区的人口将表现出生物标志物的变化，这些生物标志物检测炎症和/或基因毒性反应的方式在数量上与居住在距离有毒空气污染物主要污染源更远的人口统计学上相似的人口不同。我们将采集以下样本：1)血液样本，2)呼吸冷凝液样本，3)现场尿样。在血清中测量的生物标志物将是促炎症细胞因子、肿瘤坏死因子？、白介素6和白介素8，氧化应激测量为硫代巴比妥酸反应物质(TBARS)。预期的结果将是确定这种方法是否足够敏感，以便在与低收入和其他社会劣势特征有关的累积风险的背景下，评估暴露在社区一级的有毒空气污染物的不利影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biomarkers are biological changes that can be detected in blood, urine or other biological specimens. They can provide indications of exposure to toxic agents at the time of exposures and long before the appearance of clinical disease. There is a need to identify biomarkers that are sensitive enough to detect exposure to low levels of pollutant chemicals that are found in communities. The objective of this study to investigate the sensitivity of biomarkers that detect inflammatory and genetic toxicity responses to environmental pollutant exposures. We hypothesize that a population that resides in a community near major point sources of toxic air pollutants will express changes in biological markers that detect inflammatory and/or genotoxic responses in a quantitatively different way than a demographically similar population living in an area more distant from major sources of toxic air pollutants. We will collect the following samples 1) a blood sample 2) a breath condensate sample and 3) a spot urine sample. The biomarkers to be measured in serum will be the pro-inflammatory cytokines, TNF?, Interleukin-6, and Interleukin-8, oxidative stress measured as Thiobarbituric Acid Reactive Substances (TBARS). The anticipated outcome will be to determine whether this approach is sufficiently sensitive to be useful for the assessment of adverse effects from exposure to community level exposure to toxic air pollutants in the context of cumulative risks related to low income and other characteristics of social disadvantage.