NON-INVASIVE GLUCOSE MONITORING USING EXHALED BREATH CONDENSATE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 糖尿病患者从自我血糖监测中获益。用于执行自我葡萄糖监测的当前技术需要用刺血针刺穿皮肤以获得一滴血液。 正因为如此，它们与一些不适和感染的小风险有关。本研究测试是否可以通过测量从正常呼吸期间呼出的空气中收集的液体（这种液体称为呼出气冷凝液或EBC）中的葡萄糖水平来无创监测葡萄糖。 这一概念验证研究将分两个阶段进行。第一阶段将试图证明葡萄糖可以在EBC中测量，并且它与血糖测量相关。将使用标准非侵入性仪器在糖尿病志愿者和非糖尿病对照组中采集EBC。 将EBC葡萄糖与毛细血管血糖测量值（手指针刺法）和血糖测量值进行比较。如果该阶段成功证明可以在EBC中测量葡萄糖，并且它反映了毛细血管和血浆葡萄糖水平，则将进行第二阶段的检测。 在第二阶段，将评估EBC葡萄糖对血糖生理变化的反应。 将在糖尿病患者和非糖尿病对照组中进行标准75克口服葡萄糖耐量试验之前和期间收集EBC。 将EBC葡萄糖变化的时间过程与毛细血管和血浆葡萄糖水平的时间过程进行比较。 从本研究中收集的信息将提供可能导致开发基于EBC血糖测量原理的糖尿病患者无创血糖监测仪的信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Patients with diabetes benefit from self glucose monitoring. Current techniques for performing self glucose monitoring require piercing the skin with a lancet to obtain a droplet of blood. Because of this, they are associated with some discomfort and a small risk of infection. This study tests whether it is possible to monitor glucose non-invasively by measuring glucose levels in fluid that is collected from the air that is expired during normal breathing (this fluid is called the exhaled breath condensate or EBC). This proof of concept study will proceed in two phases. The first phase will attempt to demonstrate that glucose can be measured in EBC and that it is correlated with blood glucose measurements. EBC will be collected using a standard non-invasive apparatus in volunteers with diabetes and non-diabetic controls. EBC glucose will be compared to capillary blood glucose measurements (finger stick method) and plasma glucose measurements. If this phase successfully demonstrates that glucose can be measured in EBC and that it reflects capillary and plasma glucose levels, then the second phase of testing will be performed. In the second phase, the response of EBC glucose to physiological changes in plasma glucose will be assessed. EBC will be collected before and during a standard 75-gram oral glucose tolerance test in patients with diabetes and non-diabetic controls. The time course of changes in EBC glucose will be compared to those of capillary and plasma glucose levels. The information gathered from this study will provide information that may lead to the development of a non-invasive glucose monitor, based on the principle of EBC glucose measurements, for patients with diabetes.