National Heart, Lung, and Blood Institute Proteomics Initiative

要约人寻求应用最先进的方法，并开发新的方法， 研究心脏、肺和血液的生物过程的技术 疾病：适应缺血/缺氧。对缺氧和缺血的适应性变化是 重要的生理和变得更加突出的病理状态。因为 缺氧和缺血在受影响的组织中触发即时反应， 蛋白质组的修饰将主导最初的反应（无论是否是长期的 基因表达也发生变化）。正常和患病的详细特征 随着时间的推移，蛋白质组对缺血/缺氧的反应是理解缺血/缺氧的必要步骤。 适应机制目标是最终获得“疾病蛋白指纹”， 许多缺血性/缺氧性疾病，这可能会提供一个统一的图片， 发病机制-损伤的不同阶段的独特性和共同性 和组织类型。最先进的创新方法，加上同样先进的 和创新生物学，形成了霍普金斯建议的基石。的核心哲学 我们的中心如下：引人注目的生物学问题将推动技术创新， 蛋白质化学、仪器和数据分析。反过来，创新的应用 技术将彻底改变我们对缺血/缺氧生物学的理解。

The offeror seeks to apply state-of-the-art methods and to develop new approaches and techniques to investigate a biological process of broad interest to heart, lung and blood diseases: adaptation to ischemia/hypoxia. Adaptive changes to hypoxia and ischemia are important physiologically and become even more prominent in pathological states. Because hypoxia and ischemia trigger immediate responses in the affected tissues, post-translational modification of the proteome will dominate the initial response (whether or not longer-term changes in gene expression also occur). Detailed characterization of the normal and diseased proteomes in response to ischemia/hypoxia over time is a necessary step in understanding the adaptive mechanisms. The goal will be to eventually obtain "disease protein fingerprints" of numerous ischemic/hypoxic disorders, which may then provide a unified picture of the pathogenesis - the unique features in, and commonalities among, the various stages of injury and tissue types. State-of-the-art and innovative methods, coupled with equally state-of-the-art and innovative biology, form the cornerstone of the Hopkins proposal. The central philosophy of our Center is as follows: compelling biological questions will drive innovation in technologies of protein chemistry, instrumentation and data analysis. In turn, the application of innovative technologies will revolutionize our understanding of the biology of ischemia/hypoxia.