MODIFICATION OF A COMMON BAL TECHNIQUE TO ENHANCE SAMPLE DIAGNOSTIC VALUE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景：经支气管镜的支气管肺泡灌洗(BAL)是一种经常用于临床和研究的非人灵长类动物的诊断工具。虽然许多机构使用这一程序，但这项技术并不标准化。该程序的一个技术变体是液体回收的方法。本研究的目的是评估两种不同的BAL抽吸技术之间的差异。方法：对20只恒河猴(猕猴)进行支气管镜检查和BAL液体采集。收集用于比较的数据包括心率、血氧饱和度、直肠温度、回流液量、细胞总数、细胞活力、细胞分类计数和流式细胞仪。结果：两组患者的心率、血氧饱和度及体温均无显著差异。同样，在使用这两种方法提取的液体的细胞计数差异或细胞活性研究之间也没有统计学差异。当使用改进的抽吸技术时，全液体产量增加了8.3%，每微升液体回收的细胞数量更多。结论：增强BAL技术注意到的差异具有统计学意义，并表明与诊断有关的临床相关性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Bronchoalveolar lavage (BAL) via bronchoscopy is a diagnostic tool frequently used for clinical and research purposes in nonhuman primates. Although many institutions utilize this procedure, the technique is not standardized. One technical variant of the procedure is the method in which fluid is recovered. The purpose of this study was to evaluate differences between two different BAL aspiration techniques. Methods: Bronchoscopy and BAL fluid collection were performed on 20 rhesus macaques (Macaca mulatta). Data collected for comparison included heart rate, oxygen saturation levels, rectal temperature, volume of returned fluid, total cell count, cell viability, differential cell count, and flow cytometry. Results: Results showed no significant differences in the heart rate, SpO2 levels or body temperature between the two groups. Likewise, there were no statistical differences seen between the differential cell counts or the cell viability studies of the retrieved fluid using either method. When using the modified aspiration technique, an 8.3% increase in full fluid yield and a higher number of cells per microliter of fluid recovered were exhibited. Conclusion: The differences noted with the enhanced BAL technique are statistically significant and indicate a clinical relevance where diagnoses are concerned.