SURVEY OF ENZOOTIC PATHOGENS AND ARTHROPOD VECTORS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目是户外养殖群体健康的重要哨兵，是一项旨在评估已知地方性病原体的地方性水平的调查。从每只动物身上采集的血液和粪便样本被检查是否有感兴趣的寄生虫。粪便样本通过直接涂片法和浓缩法进行检查。血液样本通过厚薄血液涂片检查疟原虫种类和克诺特试验检查丝虫病。用酶联免疫吸附试验检测血浆中是否有克氏锥虫的证据，并保存DNA以供将来研究。潜在的媒介每周被CDC灯光陷阱和妊娠陷阱捕获。蚊子的分类和检查通过试纸ELISA法或新开发的西尼罗河病毒(WNV)LAMP试验进行。在2007年期间，我们为该项目检查了123份粪便样本和305份血液样本。今年值得注意的发现包括在一种动物身上发现了内毒素Macacai。这是一种在非霍奇金淋巴瘤中相对罕见的浆液性病变，临床上被认为是良性的。今年抽样的动物中，有整整一半(50%)携带有flleborni圆线虫，这是一种重要的病原体，并向动物护理人员提出了一系列建议，以减少这种寄生虫和鞭虫(44%)的发病率，鞭虫也是一种土传蠕虫。我们继续对怀有婴儿的母亲的乳汁进行抽样，以证明S.F.lleborni的垂直传播，但到目前为止，在任何样本中都没有检测到幼虫。这种寄生虫已被证明存在于非洲妇女的乳汁中，它在猿猴母亲的乳汁中的存在将表明可能需要开发新的治疗方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project, which serves as an important sentinel for the health of the outdoor breeding colony, is a survey designed to assess the level of endemicity of known enzootic pathogens. Samples of blood and stool obtained from each animal are examined for parasites of interest. Stool samples are examined by a direct smear and a concentration method. Blood samples are examined by thick and thin blood smears for Plasmodium species and the Knott's test for filariasis. Plasma is examined by ELISA for evidence of Trypanosoma cruzi and DNA is saved for future studies. Potential vectors are trapped weekly by CDC light traps and gravid traps. Mosquitoes are speciated and examined by dipstick ELISA test or the newly-developed LAMP test for West Nile virus (WNV). During 2007 we examined 123 stool samples and 305 blood samples for this project. Notable findings this year included Entopolypoides macacai in one animal. This is a relatively rare piroplasm in NHP and is thought to be clinically benign. Fully one half (50%) of the animals sampled this year harbored Strongyloides f¿lleborni which is an important pathogen and a series of suggestions were made to the animal care staff as to reducing the incidence of this parasite as well as Trichuris trichiura (44%) which is also a soil-transmitted helminth. We have continued our sampling of milk from mothers with babies to demonstrate vertical transmission of S. f¿lleborni but have detected no larvae in any samples so far. This parasite has been shown to be present in the milk of African women and its presence in the milk of simian mothers will be an indication that new treatment approaches may need to be developed.