SIMIAN VARICELLA VIRUS INFECTION AND LATENCY IN THE NONHUMAN PRIMATE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 猴水痘病毒（SVV）感染灵长类动物与人水痘带状疱疹病毒（VZV）感染具有相同的临床、病理、免疫学和病毒学特征。 通过将SVV血清阴性猴暴露于SVV感染的笼友猴，可在食蟹猴或非洲绿色猴中建立潜伏性SVV感染。 在这项研究中，对潜伏感染猴水痘病毒（SVV）的食蟹猴进行单次剂量（200 cGy）X射线照射，并用他克莫司（80 - 300 mg/kg/天）和泼尼松（1 mg/kg/天）治疗。 2周后，1只猴出现胸部分布带状疱疹。在其他免疫抑制猴和一个对照猴进行相同的旅行和隔离的压力，病毒学分析显示亚临床SVV再激活。因此，SVV再激活可以在潜伏感染的食蟹猴中诱导，提供了分析免疫抑制在水痘再激活中的作用的动物模型。我们正在继续研究单独测试每种药物或辐射，以测试它们在重新激活中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Simian varicella virus (SVV) infection of primates shares clinical, pathological, immunological and virological features of varicella zoster virus (VZV) infection of humans. Latent SVV infection can be established in cynomologous or African green monkeys by exposing SVV-seronegative monkeys to an SVV infected cage-mate monkey. In this study, cynomologous monkeys latently infected with simian varicella virus (SVV) were irradiated with a single dose (200 cGy) of X-irradiation and treated with tacrolimus (80-300 mg/kg/day) and prednisone (1 mg/kg/day). Two weeks later, one monkey developed thoracic distribution zoster. In the other immunosuppressed monkeys and one control monkey subjected to the same stress of travel and isolation, virological analysis revealed subclinical SVV reactivation. Thus, SVV reactivation can be induced in latently infected cynomologous macaques providing an animal model to analyze the role of immunosuppression in varicella reactivation . We are continuing studies that test each drug or irradiation alone to test their role in reactivation.