SIV T CELLS IN VIVO

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 研究免疫和未接种SIV的猕猴体内SIV特异性CD8+T细胞的功能、定位及其与体内SIV感染细胞的关系，以期对SIV的发病机制有更深入的了解。 Haase和Skinner实验室小组共同工作，确定了感染SIV的恒河猴淋巴和生殖器组织中病毒特异性CD8 T细胞与病毒感染细胞的体内效应与靶细胞比率。他们使用了早些年从这一赠款支持中开发的一种方法，称为原位四聚体染色结合原位杂交(ISTH)，并发现与淋巴组织中病毒产生细胞的减少和高效靶比有显着相关性。他们还开始通过与四聚体试剂共同标记组织来原位研究病毒特异性CD8 T细胞的表型，四聚体试剂染色病毒特异性T细胞以及穿孔素和颗粒素抗体。他们还成功地开发了用四聚体试剂和两种表型抗体进行三重标记切片的方法。对这些染色切片的分析正在进行中。此外，在这些研究过程中，他们发现了以前未描述的病毒特异性T细胞群体，它们似乎下调了B细胞滤泡以及阴道和宫颈上皮中的CD8分子。 这项工作使用了免疫学和病毒学服务。 出版物正在等待发布。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To study SIV specific CD8+ T cell function, localization, and association with SIV infected cells in vivo in vaccinated and non-vaccinated macaques in order to gain insights into SIV pathogenesis. The Haase and Skinner lab groups worked together to determine the in vivo effector to target cell ratios of virus-specific CD8 T cells to virus-infected cells in lymphoid and genital tissues of SIV-infected rhesus macaques. They did this using a methodology developed in earlier years from this grant support termed in situ tetramer staining combined with in situ hybridization (ISTH) and found a significant correlation with reduction in virus-producing cells in lymphoid tissues and high effector to target ratios. They also began to investigate the phenotype of virus-specific CD8 T cells in situ by co-labeling tissues with tetramer reagents that stain virus-specific T cells and perforin and granulin antibodies. They also were successful in developing methods to triple labeling sections with tetramer reagents and two phenotypic antibodies. Analysis of these stained sections is ongoing. In addition, during the course of these studies they discovered previously undescribed populations of virus-specific T cells that appear to down modulate CD8 molecules in B cell follicles and in vaginal and cervical epithelium. This work used Immunology & Virology Services. Publications are pending.