MICROTUBULE DYNAMICS IN YEAST

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 Odde实验室（明尼苏达大学）有兴趣在运动功能和微管动力学相互作用的水平上了解酵母中的有丝分裂纺锤体，以便可以数学模型有丝分裂纺锤体。 他们最初的建模工作在（Gardner M.K.等（2005）在动粒处微管动力学的张力依赖性调节可以解释酵母中的中期聚集。分子生物学细胞16：3764-3775）。 为了建立这个数学模型，他们使用了来自Boulder实验室的关于酵母有丝分裂纺锤体的先前3D超微结构数据（Winey，M.，等（1995）酿酒酵母有丝分裂纺锤体的三维超微结构分析。细胞生物学杂志129：1601-1616。）。 为了扩展他们的建模并通过使用它来预测酵母菌株中各种纺锤体组分突变体中纺锤体的行为来测试模型，Dave Odde安排了他的团队（数学建模）与Kerry Bloom（北卡罗来纳州-查佩尔山，活细胞成像）和Mark Winey和Boulder Lab之间的合作，用于细胞的3D结构（EM断层扫描）。 到目前为止，我们已经重新审视了野生型纺锤体结构，使用断层扫描，以确认以前的结果的基础上重建从连续薄切片，并作为控制突变体的分析。 我们也一直在收集数据集，并从缺乏CIN 8驱动蛋白样马达的突变株中建模纺锤体，这非常有趣。 这项工作证明是非常富有成效的，并可能导致今年的手稿。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Odde lab (Univ. Minnesota) is interested in understanding the mitotic spindle in yeast at the level of interplay of motor function and microtubule dynamics such that the can mathematical model mitotic spindles. Their initial modeling work is presented in (Gardner M.K. et al. (2005) Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast. Mol Biol Cell. 16:3764-3775.). To build this mathematical model they used previous 3D ultrastructural data on yeast mitotic spindles from the Boulder Laboratory (Winey, M., et al. (1995) Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle. J. Cell Biol. 129:1601-1616.). To expand their modeling and to test the model by using it to predict the behavior of spindles in yeast strains mutant in various spindle components, Dave Odde has arranged a collaboration between his group (mathematical modeling) and that of Kerry Bloom (Univ. North Carolina - Chapel Hill, live-cell imaging) and Mark Winey and the Boulder Lab for the 3D Structure of Cells (EM tomography). Thus far, we have revisited wild-type spindle structure using tomography to confirm the previous results based on reconstruction from serial thin sections and to serve as controls for the analysis of mutants. We have also been collecting datasets and modeling spindles from a mutant strain lacking the CIN8 kinesin-like motor, which are very interesting. This work is proving very fruitful and may lead to a manuscript this year.