MICROTUBULE DYNAMICS IN YEAST

酵母中的微管动力学

• 批准号: 8170838
• 负责人: David J. Odde
• 金额: $ 1.87万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170838
• 关键词: Behavior; Cells; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; Funding; Grant; Institution; Kinesin; Kinetochores; Laboratories; Lead; Life; Manuscripts; Metaphase; Microtomy; Microtubules; Minnesota; Mitotic spindle; Modeling; Motor; North Carolina; Regulation; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Source; Structure; Testing; United States National Institutes of Health; Work; Yeasts; base; cellular imaging; interest; mathematical model; mutant; reconstruction; three dimensional structure; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Odde lab (Univ. Minnesota) is interested in understanding the mitotic spindle in yeast at the level of interplay of motor function and microtubule dynamics such that the can mathematical model mitotic spindles. Their initial modeling work is presented in (Gardner M.K. et al. (2005) Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast. Mol Biol Cell. 16:3764-3775.). To build this mathematical model they used previous 3D ultrastructural data on yeast mitotic spindles from the Boulder Laboratory (Winey, M., et al. (1995) Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle. J. Cell Biol. 129:1601-1616.). To expand their modeling and to test the model by using it to predict the behavior of spindles in yeast strains mutant in various spindle components, Dave Odde has arranged a collaboration between his group (mathematical modeling) and that of Kerry Bloom (Univ. North Carolina - Chapel Hill, live-cell imaging) and Mark Winey and the Boulder Lab for the 3D Structure of Cells (EM tomography). Thus far, we have revisited wild-type spindle structure using tomography to confirm the previous results based on reconstruction from serial thin sections and to serve as controls for the analysis of mutants. We have also been collecting datasets and modeling spindles from a mutant strain lacking the CIN8 kinesin-like motor, which are very interesting. This work is proving very fruitful and may lead to a manuscript this year.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 奥德实验室（明尼苏达大学）有兴趣在运动功能和微管动力学相互作用的水平上了解酵母的有丝分裂纺锤体，以便可以对有丝分裂纺锤体进行数学模型。 他们最初的建模工作发表于（Gardner M.K. et al. (2005) Tension-dependent adjustment of microtubuledynamics at kinetochores can treat mephase congression in酵母。Mol Biol Cell. 16:3764-3775.）。 为了建立这个数学模型，他们使用了 Boulder 实验室先前关于酵母有丝分裂纺锤体的 3D 超微结构数据（Winey, M., et al. (1995) Three-Dimensional ultrastructuralanalysis of the Saccharomyces cerevisiae mitotic纺锤体。J. Cell Biol. 129:1601-1616.）。 为了扩展他们的模型并通过使用它来预测酵母菌株突变体中各种纺锤体成分的纺锤体行为来测试模型，Dave Odde 安排了他的团队（数学建模）与 Kerry Bloom 团队（北卡罗来纳大学教堂山分校，活细胞成像）以及 Mark Winey 和博尔德实验室进行细胞 3D 结构（EM 断层扫描）的合作。 到目前为止，我们已经使用断层扫描重新审视了野生型纺锤体结构，以确认之前基于连续薄片重建的结果，并作为突变体分析的对照。 我们还一直在收集缺乏 CIN8 类驱动蛋白马达的突变株的数据集和纺锤体模型，这非常有趣。 事实证明，这项工作非常富有成果，今年可能会形成手稿。