Analytical Neurochemistry and Spectroscopy Core

这个核心的目标是：(1)测量对大脑化学至关重要的“小分子”(主要是氨基酸和生物胺)。(2)用质谱仪测定稳定同位素富集度，以便在体外和发育障碍人群中进行代谢通量的动力学研究。(3)鉴定和鉴定对神经功能有重要作用的多肽和蛋白质。(4)进行以细胞为基础的对大脑功能重要的生理过程的分析，包括钙离子、钙蛋白、半胱氨酸天冬氨酸氨基转移酶和 ROS；(5)协助用户进行实验设计和数据解释。 核心的理论基础是，神经化学紊乱发生在许多发育障碍中，包括代谢性疾病、缺氧性损伤、癫痫、神经毒性等。

The goals of this Core are to: (1) Measure "small molecules" (primarily amino acids and biogenic amines) of central importance to brain chemistry. (2) Assay stable isotope enrichment with mass spectrometry in order to perform kinetic studies of metabolic flux, both in vitro and in people with developmental disabilifies. (3) Idenfify and characterize pepfides and proteins that are important to neurologic function. (4) Perform cell-based assays of physiologic processes that are important to brain funcfion, including assays of Ca2+, calpain, caspase and ROS; (5) Assist users with regard to experimental design and data interpretation. The rationale for the core is that neurochemical derangements occur in many developmental disabilifies, including metabolic diseases, hypoxic injury, epilepsy, neurotoxicity, etc.