SIV T CELLS IN VIVO

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：研究免疫和未免疫猕猴体内SIV特异性CD 8 + T细胞的功能、定位以及与SIV感染细胞的相关性，以深入了解SIV的发病机制。 Haase和Skinner实验室小组共同确定了SIV感染恒河猴淋巴和生殖组织中病毒特异性CD 8 T细胞与病毒感染细胞的体内效应细胞与靶细胞比率。 他们使用了早些年从该资助中开发的方法，称为原位四聚体染色结合原位杂交（ISTH），并发现与淋巴组织中病毒产生细胞的减少和高效应靶比显着相关。他们还开始研究病毒特异性CD 8 T细胞的表型，方法是用染色病毒特异性T细胞的四聚体试剂以及穿孔素和颗粒蛋白抗体共标记组织。 他们还成功地开发了用四聚体试剂和两种表型抗体三重标记切片的方法。 对这些染色切片的分析正在进行中。 此外，在这些研究过程中，他们发现了以前未描述的病毒特异性T细胞群体，这些细胞似乎下调了B细胞卵泡以及阴道和宫颈上皮中的CD 8分子。 这项工作使用免疫学和病毒学服务。 出版物待定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To study SIV specific CD8+ T cell function, localization, and association with SIV infected cells in vivo in vaccinated and non-vaccinated macaques in order to gain insights into SIV pathogenesis. The Haase and Skinner lab groups worked together to determine the in vivo effector to target cell ratios of virus-specific CD8 T cells to virus-infected cells in lymphoid and genital tissues of SIV-infected rhesus macaques. They did this using a methodology developed in earlier years from this grant support termed in situ tetramer staining combined with in situ hybridization (ISTH) and found a significant correlation with reduction in virus-producing cells in lymphoid tissues and high effector to target ratios. They also began to investigate the phenotype of virus-specific CD8 T cells in situ by co-labeling tissues with tetramer reagents that stain virus-specific T cells and perforin and granulin antibodies. They also were successful in developing methods to triple labeling sections with tetramer reagents and two phenotypic antibodies. Analysis of these stained sections is ongoing. In addition, during the course of these studies they discovered previously undescribed populations of virus-specific T cells that appear to down modulate CD8 molecules in B cell follicles and in vaginal and cervical epithelium. This work used Immunology & Virology Services. Publications are pending.