MEASLES VIRUSES WITH ADDED VACCINE SPECIFICITIES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 麻疹减毒活疫苗具有突出的效率和安全记录，并诱导持久的免疫力。我们假设，目前使用的麻疹病毒（MV）疫苗株是一个理想的平台，为儿童疫苗的发展，引发对其他病原体的免疫力。我们将测试这个假设的两个命题：首先，载体MV可以防止感染另一种病原体。第二，它们可以引发针对其他人类病原体的强烈免疫应答，同时保持针对麻疹的疫苗功能。以不同水平表达B型肝炎病毒表面抗原的重组MV将用于确定诱导强体液免疫应答的最有效表达策略。将在转基因小鼠中诱导最强抗B肝炎免疫应答的那些病毒接种到幼年猕猴中。将测量它们在保护这些灵长类动物免受野生型麻疹感染和诱导针对额外病原体的免疫应答方面的功效。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The live attenuated measles vaccine has an outstanding efficiency and safety record and induces enduring immunity. We hypothesize that a currently used measles virus (MV) vaccine strain is an ideal platform for the development of pediatric vaccines eliciting immunity against other pathogens. We will test two propositions of this hypothesis: first that vectored MVs can protect against infection with another pathogen. Second, that they can elicit a strong immune response against other human pathogens whilst maintaining vaccine function against measles. Recombinant MV expressing the surface antigen of the hepatitis B virus at different levels will be used to define the expression strategy most effective in inducing a strong humoral immune response. Those viruses inducing the strongest immune response against hepatitis B in genetically modified mice will be inoculated into juvenile macaques. Their efficacy in protecting these primates against wild type measles infection and in inducing an immune response against the additional pathogen will be measured.