GENES CONTROLLING PUBERTY ONSET

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 目的：探讨控制青春发育时间的基因。 了解大脑中青春期的机制是很重要的，因为一些疾病和行为障碍与青春期有关。在这项研究中，Kisspeptin在青春期LHRH释放增加的作用进行了研究。结果表明，kisspeptin-54释放的茎正中隆起的微透析方法测量是脉动的，约75%的kisspeptin-54脉冲与LHRH脉冲。此外，kisspeptin-10刺激LHRH释放的剂量依赖性的方式。最后，kisspeptin-54在通过推拉灌注方法收集的“存档样品”中进行测量，其中LHRH水平先前已经测量。结果表明，Kisspeptin-54释放的显着增加与青春期LHRH-1释放的增加有关，并且在青春期前的猴子中已经观察到Kisspeptin-54释放的夜间增加，并持续到青春期。夜间增加LHRH-1释放发生在青春期早期和青春期中期，但不是在青春期前的猴子。总的来说，研究结果与kisspeptin在青春期发挥作用的假设一致。本研究使用WNPRC Animal Services和Assay Services。 了解大脑中青春期的机制是很重要的，因为一些疾病和行为障碍与青春期有关。在这项研究中，Kisspeptin在青春期LHRH释放增加的作用进行了研究。结果表明，kisspeptin-54释放的茎正中隆起的微透析方法测量是脉动的，约75%的kisspeptin-54脉冲与LHRH脉冲。此外，kisspeptin-54在通过推拉灌注方法收集的“存档样品”中测量，其中LHRH水平先前已测量。结果表明，Kisspeptin-54释放的显着增加与青春期LHRH-1释放的增加有关，并且在青春期前的猴子中已经观察到Kisspeptin-54释放的夜间增加，并持续到青春期。夜间增加LHRH-1释放发生在青春期早期和青春期中期，但不是在青春期前的猴子。最后，kisspeptin-10刺激LHRH释放在青春期前和青春期的猴子以剂量依赖的方式。总的来说，研究结果与kisspeptin在青春期发挥作用的假设一致。本研究使用WNPRC Animal Services和Assay Services。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To investigate genes controlling the timing of puberty. Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. In this study the role of kisspeptin in the pubertal increase in LHRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile, and approximately 75% of kisspeptin-54 pulses were correlated with LHRH pulses. Moreover, kisspeptin-10 stimulated LHRH release in a dose-dependent manner. Finally, kisspeptin-54 was measured in "archived samples" collected by a push-pull perfusion method, in which LHRH levels had been previously measured. The results indicate that a significant increase in kisspeptin-54 release occurred in association with the pubertal increase in LHRH-1 release and that a nocturnal increase in kisspeptin-54 release was already observed in prepubertal monkeys and continued through the pubertal period. A nocturnal increase in LHRH-1 release occurred in early pubertal and midpubertal, but not in prepubertal monkeys. Collectively, the results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services. Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. In this study the role of kisspeptin in the pubertal increase in LHRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile, and approximately 75% of kisspeptin-54 pulses were correlated with LHRH pulses. Moreover, kisspeptin-54 was measured in "archived samples" collected by a push-pull perfusion method, in which LHRH levels had been previously measured. The results indicate that a significant increase in kisspeptin-54 release occurred in association with the pubertal increase in LHRH-1 release and that a nocturnal increase in kisspeptin-54 release was already observed in prepubertal monkeys and continued through the pubertal period. A nocturnal increase in LHRH-1 release occurred in early pubertal and midpubertal, but not in prepubertal monkeys. Finally, kisspeptin-10 stimulated LHRH release in a dose-dependent manner in both prepubertal and pubertal monkeys. Collectively, the results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services.